Selected CEITEC Publications

We would like to regularly present selected publications written with the participation of CEITEC scientists, which we believe cover thought-provoking topics. We hope you find these topics interesting.Titles of publications are linked to complete versions.

TRAC – Trends in Analytical Chemistry

Assays for determination of matrix metalloproteinases and their activity
Krizkova, S.; Zitka, O.; Masarik, M.; Adam, V.; Stiborova, M.; Eckschlager, T.; Chavis, G. J.; Kizek, R.

CEITEC Research Group: Submicron Systems and Nanodevices
Research Programme 1: Advanced Nanotechnologies and Microtechnologies

Summary
Matrix metalloproteinases (MMPs) are involved in many physiological and pathological processes. Numerous MMPs assays were developed for both clinical and research purposes, but far more attention was turned to understanding their biological functions. In this review, enzymatic, immunochemical and fluorimetric methods as well as in vivo imaging methods are discussed. Moreover, we aimed our attention on additional methods that are now subject to investigation, such as phage display, Multiple-Enzyme/Multiple-Reagent Assay System (MEMRAS) and activity based profiling.

Applied Physics Letters

In-situ observation of 110 oriented Ge nanowire growth and associated collector droplet behavior
Kolibal, M.; Vystavel, T.; Novak,L; Mach,J.; Sikola, T.

CEITEC Research Group: Fabrication and Characterization of Nanostructures
Research Programme 1: Advanced Nanotechnologies and Microtechnologies

Summary
The article reports on the growth of <110> oriented germanium nanowires in a wide range of their diameters. The explanation given in the paper shows why only the nanowires with very small diameters growing in this orientation were obtained so far. The control of the nanowire orientation is crucial for their application in sensorics and novel nanowire-based electronics.

Journal of Nuclear Materials

Fatigue cracks in Eurofer 97 steel: Part I. and  II.
Kruml, T.;  Polak, J.; Hutar, P.;  Nahlik, L.;  Seitl, S.

CEITEC Research Group: Advanced Metallic Materials and Metal Based Composites
Research Programme 2: Advanced Materials

Summary
The Eurofer 97 steel belongs to the family of high chromium ferritic–martensitic steels. It is a candidate structural material for the future nuclear fusion programme. It is supposed to be in service under intense irradiation, therefore a care is taken to use only chemical elements with fast decay properties. The fatigue crack growth rate in the Eurofer 97 steel was measured by two different methodologies and law characterizing the small fatigue crack behaviour was described in the works. Results obtained can be used for the residual fatigue life prediction based on the small crack growth kinetics.

Polymer

Molecular weight scaling of the spherulite growth rate in isothermally melt crystallized polyethylene nanocomposites
Jancar, J.; Fiore, K.

CEITEC Research Group: Advanced Polymers and Composites
Research Programme 2: Advanced Materials

Summary
In this paper, the effect of adding small amount of high specific surface area fumed silica on the crystallization kinetics of three monodisperse polyethylenes crystallized from quiescent melt at small undercooling was investigated. Linear spherulite growth rate under temperature regime II, GII, was measured employing the hot-stage and optical microscope with polarized light equipped with color plate. Experimental data were analyzed using the modified LH secondary nucleation theory attempting to identify the specific molecular mechanism correlating with the observed variations of the activation energy of the surface transport term with varying Mn and SiO2 nanoparticle content.

Developmental Cell

Cytokinin Modulates Endocytic Trafficking of PIN1 Auxin Efflux Carrier to Control Plant Organogenesis
Marhavy, P.; Bielach, A.; Abas, L.; Abuzeineh, A.; Duclercq, J.; Tanaka, H.; Parezova, M.; Petrasek, J.; Friml, J.; Kleine-Vehn, J.; Benkova, E.

CEITEC Research Group: Hormonal Crosstalk in Plant Development
Research Programme 4: Genomics and Proteomics of Plant Systems

Summary
In the article we show that cytokinin might modulate auxin efflux not only on the level of transcription, but also posttranscriptionally. At increased cytokinin levels one of the auxin efflux carriers, namely PIN1 is redirected for rapid degradation to lytic vacuoles. We identified an alternative mode of cytokinin action that uses endocytic trafficking as a means to direct plant organogenesis.

The Plant Journal

Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana
Pekarova, B.; Klumpler, T.; Triskova, O.; Horak, J.; Jansen, S.; Dopitova, R.; Borkovcova, P.; Papouskova, V.; Nejedla, E.; Sklenar, V.; Marek, J.; Zidek, L.; Hejatko, J.; Janda, L.

CEITEC Research Group: Functional Genomics and Proteomics of Plants
Research Programme 4: Genomics and Proteomics of Plant Systems

Summary
We have shown that the receiver domain of sensor histidine kinase is necessary and sufficient for the specific interaction with subset of its downstream signalling partners, AHP proteins in Arabidopsis. Further, we determined the crystal structure of the receiver domain and identified the magnesium-mediated structural changes with impact on the binding specificity.

The Plant Journal

Polarization of PIN3-dependent auxin transport for hypocotyls gravitropic response in Arabidopsis thaliana
Rakusova, H.; Gallego-Bartolome, J.; Vanstraelen, M.; Robert, H.S.;  Alabadı, D.; Blazquez, M.A.; Benkova, E.; Friml, J.

CEITEC Research Group: Developmental and Cell Biology of Plants
Research Programme 4: Genomics and Proteomics of Plant Systems Research Programme

Summary
In this paper we provide insights into the mechanism for hypocotyl gravitropic growth in Arabidopsis thaliana. We show that gravity perception polarizes PIN3 auxin transporter to the lower side of endodermis cells to mediate asymmetric auxin distribution for gravitropic shoot bending.

Journal of Clinical Oncology

Missense mutations located in structural p53 DNA-binding motifs are associated with extremely poor survival in chronic lymphocytic leukemia.

CEITEC Research Group: Molecular Oncology I – Hematooncology
Research Programme 5: Molecular Medicine

Summary
There is a distinct connection between TP53 defects and poor prognosis in chronic lymphocytic leukemia (CLL). It remains unclear whether patients harboring TP53 mutations represent a homogenous prognostic group. We evaluated the survival of patients with CLL and p53 defects identified at our institution by p53 yeast functional assay and complementary interphase fluorescence in situ hybridization analysis detecting del (17p) from 2003 to 2010.

International Journal  of Neuropsychopharmacol

The effects of methamphetamine self-administration on behavioural sensitization in the olfactory bulbectomy rat model of depression
Kucerova, J.; Pistovcakova, J.; Vrskova, D.; Dusek, L.; Sulcova, A.

CEITEC Research Group: Experimental and Applied Neuropsychopharmacology
Research Programme 6: Brain and Mind Research

Summary
Use of olfactory bulbectomy model of  depression and IV self-administration paradigm was validated as a suitable  solution to simulate co-morbidity of drug addiction and depression in  experimental rodents.  The study has proven that animal model of depression increases methamphetamine intake in the model of drug addiction. Furthermore, increasing efficacy of methamphetamine was registered during repeated administration (sensitization to drug effects).

Journal of Antimicrobial Chemotherapy

CTX-M-15-producing Escherichia coli clone B2-O25b-ST131 and Klebsiella spp. isolates in municipal wastewater treatment plant effluents
Dolejska, M.; Frolkova, P.;  Florek, M.; Jamborova, I.; Purgertova, M.; Kutilova, I.; Cizek, A.; Guenther, S.; Literak, I.

CEITEC Research Group: Molecular Bacteriology
Research Programme 7: Molecular Veterinary Medicine

Summary
The global occurrence of antibiotic resistance genes in bacteria in water environments is an increasing concern. The results of the study highlight the inadequacy of the treatment process in removing multiresistant bacteria from municipal wastewater and point to a risk of transmission of clinically important multiresistant strains, such as the pandemic ST131 clone, to the environment. This is the first study demonstrating the pandemic ST131 clone in wastewater.