Změnit instituci
Pokročilé nano a mikrotechnologie Pokročilé materiály Strukturní biologie Gen. a prot. rostlin. systémů Molekulární medicína Výzkum mozku a lidské mysli Molekulární vet. medicína

Vývojová a produkční biologie - omické přístupy

Vedoucí výzkumné skupiny
Researcher ID
E-mail:
Kancelář:

Research areas

  • Molecular mechanisms underlying:
  1. hormonal regulation of plant development
  2. plant adaptation to abiotic and biotic cues
  • Molecular markers related to economically important traits

Main objectives

Determination of molecular mechanisms governing hormonal regulations and their functions in plant development and stress-response. Developmental outputs of cell polarity will be established.

Content of research

Hormonal control of plant growth correlations and responses to environmental cues

Throughout their life cycle, plants as sessile organisms depend critically on sensing a number of external and internal signals that they use to alter their physiology, morphology and development. Plant hormones, mainly auxin and cytokinin, have been recognised as key players in the control of plant growth correlations, and an increasing body of evidence implicates their involvement in the responses to environmental cues. We will investigate the molecular and cellular mechanisms underlying the hormonal modulation of the plant body architecture, mainly shoot and root branching, under various environmental cues including nutrient and/or water deficiency. Our pilot experiments indicate that alterations in the hormone level or signalling can compensate for the undesirable effects of the environmental cues on plant growth and morphogenesis. We will develop and employ reverse genetic tools to get a deeper understanding of the particular roles of a number of candidate genes implicated in the compensation responses. In addition, green fluorescent protein (GFP) fusions will be used to monitor promoter activities and protein products of genes reportedly involved in branching, for example genes involved in the maintenance and differentiation of the axillary meristems and auxin transport. Based on the knowledge gained in the project we will evaluate the possibilities of crop engineering for better performance under limited nutrient and water availability.

Much progress has been made recently in our understanding of cytokinin biosynthesis and signaling, and how environmental cues interact with these components to modulate plant growth, development and physiology. Cytokinin probably plays a role in the response to many environmental signals. Physiological studies have correlated the changes in cytokinin levels in response to environmental cues. Genome-wide microarray studies reveal overlapping transcriptional responses between cytokinin and various environmental inputs. The components of the cytokinin biosynthetic and signaling pathway are, in turn, transcriptionally altered by environmental stimuli. While many of the links of cytokinin to various environmental stimuli have been simply correlative, recent studies using mutants that alter cytokinin biosynthesis or signalling have begun to demonstrate an important role for cytokinins in these responses. However, because of the wide range of outputs of the cytokinin signalling pathway, dissecting the role of cytokinin in the response to a particular stress remains challenging. We will employ a number of tools now available to alter cytokinin levels and responsiveness in combination with non targeted transcriptomic and proteomic analyses to deepen our knowledge of this subject. The investigation of structure-function relationships in proteins involved in these interactions will provide us with knowledge of their mechanisms of action at the molecular level. We will focus on the interactions of cytokinin and the light, temperature and drought response pathways. We will also examine the effects of altered cytokinin levels and signalling on the accumulation of and tolerance to heavy metals. Attention will be paid to cytokinin interactions with other hormones in these processes. As we further deepen our understanding of the circuitry underlying the input of cytokinin into the response of the environmental signals, we should be able to engineer these pathways to produce plants with increased tolerance to abiotic stresses.

seznam / vizitky

Jméno a pozice

E-mail

Telefon

Bc. Jan Skalák
Ing. Jozef Balla, Ph.D.
prof. RNDr. Břetislav Brzobohatý, CSc.
Mgr. Martin Černý
Ing. Pavel Hanáček, Ph.D.
prof. RNDr. Aleš Knoll, Ph.D.
Senior researcher
Mgr. Alena Kuklová
Mgr. Lea Lojková, Ph.D.
Mgr. Pavel Mazura, Ph.D.
Ing. Zuzana Medvěďová
prof. Ing. Stanislav Procházka, DrSc.
Andrea Tripepi
doc. Ing. Tomáš Urban, Ph.D.
Ing. Michaela Vacková
Technická pracovnice pro vědu a výzkum
Mgr. Pavlína Váňová
Ing. Zuzana Vránová
Ing. Tomáš Vyhnánek, Ph.D.
Ing. Filip Weisz, Ph.D.
Mgr. Dušan Turek

VYBRANÉ PUBLIKACE

2016

  • BALLA, J; MEDVED'OVA, Z; KALOUSEK, P; MATIJESCUKOVA, N; FRIML, J; REINOHL, V; PROCHAZKA, S, 2016:Auxin flow-mediated competition between axillary buds to restore apical dominance. SCIENTIFIC REPORTS 6
  • HABANOVA, H; BRZOBOHATY, B; CERNY, M, 2016:The development of barley seed protein library for targeted analyses. FEBS JOURNAL 283 , p. 326 - 326.
  • SKALAK, J; CERNY, M; JEDELSKY, P; DOBRA, J; GE, E; NOVAK, J; HRONKOVA, M; DOBREV, P; VANKOVA, R; BRZOBOHATY, B, 2016:Stimulation of ipt overexpression as a tool to elucidate the role of cytokinins in high temperature responses of Arabidopsis thaliana. JOURNAL OF EXPERIMENTAL BOTANY 67 (9), p. 2861 - 2873.

2015

  • DOBRA, J; CERNY, M; STORCHOVA, H; DOBREV, P; SKALAK, J; JEDELSKY, PL; LUKSANOVA, H; GAUDINOVA, A; PESEK, B; MALBECK, J; VANEK, T; BRZOBOHATY, B; VANKOVA, R, 2015:The impact of heat stress targeting on the hormonal and transcriptomic response in Arabidopsis. PLANT SCIENCE 231 , p. 52 - 61.
  • NOVAK, J; CERNY, M; PAVLU, J; ZEMANKOVA, J; SKALAK, J; PLACKOVA, L; BRZOBOHATY, B, 2015:Roles of Proteome Dynamics and Cytokinin Signaling in Root to Hypocotyl Ratio Changes Induced by Shading Roots of Arabidopsis Seedlings. PLANT AND CELL PHYSIOLOGY 56 (5), p. 1006 - 1018.

2014

  • BORKOVCOVA, P; PEKAROVA, B; VALKOVA, M; DOPITOVA, R; BRZOBOHATY, B; JANDA, L; HEJATKO, J, 2014:Antibodies against CKI1(RD), a receiver domain of the sensor histidine kinase in Arabidopsis thaliana: From antigen preparation to in planta immunolocalization. PHYTOCHEMISTRY 100 , p. 6 - 15.
  • BRUYNS, PV; KLAK, C; HANACEK, P, 2014:Evolution of the stapeliads (Apocynaceae-Asclepiadoideae) - repeated major radiation across Africa in an Old World group. MOLECULAR PHYLOGENETICS AND EVOLUTION 77 , p. 251 - 263.
  • CERNY, M; JEDELSKY, PL; NOVAK, J; SCHLOSSER, A; BRZOBOHATY, B, 2014:Cytokinin modulates proteomic, transcriptomic and growth responses to temperature shocks in Arabidopsis. PLANT CELL AND ENVIRONMENT 37 (7), p. 1641 - 1655.
  • KONECNA, E; SAFAROVA, D; NAVRATIL, M; HANACEK, P; COYNE, C; FLAVELL, A; VISHNYAKOVA, M; AMBROSE, M; REDDEN, R; SMYKAL, P, 2014:Geographical Gradient of the eIF4E Alleles Conferring Resistance to Potyviruses in Pea (Pisum) Germplasm. PLOS ONE 9 (3)
  • TUREK, D; KLIMES, P; MAZURA, P; BRZOBOHATY, B, 2014:Combining Rational and Random Strategies in beta-Glucosidase Zm-p60.1 Protein Library Construction. PLOS ONE 9 (9)

2013

  • CEPICA, S; ZAMBONELLI, P; WEISZ, F; BIGI, M; KNOLL, A; VYKOUKALOVA, Z; MASOPUST, M; GALLO, M; BUTTAZZONI, L; DAVOLI, R, 2013:Association mapping of quantitative trait loci for carcass and meat quality traits at the central part of chromosome 2 in Italian Large White pigs. MEAT SCIENCE 95 (2), p. 368 - 375.
  • CERNY, M; KUKLOVA, A; HOEHENWARTER, W; FRAGNER, L; NOVAK, O; ROTKOVA, G; JEDELSKY, PL; ZAKOVA, K; SMEHILOVA, M; STRNAD, M; WECKWERTH, W; BRZOBOHATY, B, 2013:Proteome and metabolome profiling of cytokinin action in Arabidopsis identifying both distinct and similar responses to cytokinin down- and up-regulation >. JOURNAL OF EXPERIMENTAL BOTANY 64 (14), p. 4193 - 4206.
  • CERNY, M; SKALAK, J; CERNA, H; BRZOBOHATY, B, 2013:Advances in purification and separation of posttranslationally modified proteins. JOURNAL OF PROTEOMICS 92 , p. 2 - 27.

GRANTY

  • Modus operandi cytokinin-vnímajících histidin kinas v rostlinách (15-19266S), Czech Science Foundation - Standard Grants, 2015 - 2017
  • Proteome dynamics in response to increased cytokinin levels in Arabidopsis (IAA600040701), Academy of Sciences of the Czech Republic - Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Rep., 2007 - 2010
  • The role of cytokinins and polyamines in heat stress response and thermotolerance in tobacco and Arabidopsis plants (GA206/09/2062), Czech Science Foundation - Standard Grants, 2009 - 2013
  • Application of conventional and molecular genetic approaches for the development of grain legumes resistant to viral and fungal pathogens and insect pests (QI91A229), Ministry of Agriculture - The Research Program in the Agricultural Sector, 2009 - 2013
  • Functional genomics and proteomics for crop improvement (1M06030), MEYS - Institutional Research Plan, 2006 - 2011
  • Regulation of morphogenesis of plant cells and organs (LC06034), MEYS - Basic Research Centres, 2006 - 2011

CURRENT RESEARCH INFRASTRUCTURE

The current research infrastructure of the group includes plant cultivation facilities, microscopy, molecular biology and biochemistry equipment.

1. Plant Responses to Combined Abiotic Stresses – Omics Perspective

Supervisor: prof. RNDr. Břetislav Brzobohatý, CSc.

Annotation:

Sustaining agricultural production in a rapidly changing climate requires a detailed knowledge of the molecular basis of plant-environment interactions. As hormones play a crucial role in modulating plant responses to environmental cues, hormonal control of plant responses to environmental cues is receiving ever growing attention. A key role of a plant hormone cytokinin in these modulations is emerging. While components of signalling circuits of cytokinin and light have been largely uncovered, those of other environmental factors, e.g. temperature and drought, are only being elucidated. Consequently, our knowledge of mutual interactions of cytokinin and combined abiotic stresses, and their projection into particular phenotypes represents an area of intense current and future research. A number of tools now available to alter cytokinin levels and responsiveness in combination with transcriptomics, proteomics, metabolomics and phenomics analyses will be employed to deepen our knowledge of this subject. Work will focus mainly on the interactions of cytokinin, temperature and drought pathways. In future, the knowledge obtained is expected to be employed to engineer plants with desired responses to environmental cues via synthetic biology approaches.

Přečtěte si také
Educational Course: NeuroImaging - Mapping the function and structure of brain

19. září 2017 14:37

Educational Course: NeuroImaging - Mapping the function and structure…

WHEN: November 14-16, 2017 WHERE: CF MAFIL, CEITEC MU, University Campus Bohunice, Brno

Mladá laborantka z CEITEC VUT zvítězila v mezinárodní posterové soutěži

19. září 2017 9:15

Mladá laborantka z CEITEC VUT zvítězila v mezinárodní posterové…

Markéta Tesařová je sice teprve v magisterském studiu oboru Fyzikální inženýrství a nanotechnologie FSI VUT, ale už sbírá úspěchy i v oblasti…