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Centrální laboratoř - Proteomika

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Head of Core Facility
Researcher ID
Telefon: +420 54949 8258
Mobil: 777926602
E-mail: ,
Kancelář:

Main Activity

Mass spectrometry – based proteomics. Activities of the Core Facility cover all steps of proteomic analysis – protein isolation, separation of protein mixtures, protein characterisation by mass spectrometry and bioinformatic data processing.

Unique Features

The Core Facility provides academic community and other subjects with access to advanced proteomic technologies based on shared resources and highly trained staff. The concentration of expensive instrumentation and know-how results in fast responses to the demands of the research community and the effective utilization of resources.

Key Equipment (Core Facility fully operational from 2014)

  • High-resolution mass spectrometer (FTMS)
  • Hybrid mass spectrometer (e.g. Q-LIT)
  • MALDI-TOF/TOF mass spectrometer

The Core Facility is part of Czech National Affiliated Centre of INSTRUCT.

All CEITEC core facilities are available to external users (academia and companies). Czech and international researchers from universities and research institutes interested in accessing core facilities can benefit from support of research infrastructure CIISB, funded by the Ministry of Education, Youth and Sports of the Czech Republic.

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seznam / vizitky

Jméno a pozice

E-mail

Telefon

doc. RNDr. Zbyněk Zdráhal, Dr.
Vedoucí výzkumné skupiny
+420 54949 8258
Mgr. David Potěšil, Ph.D.
Výzkumný pracovník
+420 54949 8426, +420 54949 8428
RNDr. Hana Konečná
Výzkumný pracovník
+420 54949 5050
Mgr. Ondrej Šedo, Ph.D.
Výzkumný pracovník
+420 54949 7304, +420 54949 8427
Mgr. Michal Obořil
PhD student
Ing. Martina Grochalová
PhD student
+420 54949 4721
RNDr. Ivana Ihnatová
Externí spolupráce
+420 54949 8442
Danuše Fridrichová
Laborant
+420 54949 8425
Mgr. Ondřej Bočánek
Odborný pracovník
+420 54949 8442
Mgr. Eva Srbová
Odborný pracovník
Mgr. Kamil Mikulášek
+420 54949 8442
Mgr. Kateřina Hanáková
PhD student
+420 54949 8442
Mgr. Martin Bednařík
RNDr. Erika Lattová, PhD.
Výzkumný pracovník
+420 54949 8425
Sri Ranjani Ganji, Ph.D., M.Sc.

VYBRANÉ PUBLIKACE

2017

  • BOČÁNEK, O; ŠEDO, O; PEKÁR, S; ZDRÁHAL, Z, 2017:Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS. TOXICON 133 , p. 18 - 25.

2016

  • CERVENKA, I; VALNOHOVA, J; BERNATIK, O; HARNOS, J; RADSETOULAL, M; SEDOVA, K; HANAKOVA, K; POTESIL, D; SEDLACKOVA, M; SALASOVA, A; STEINHART, Z; ANGERS, S; SCHULTE, G; HAMPL, A; ZDRAHAL, Z; BRYJA, V, 2016:Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113 (33), p. 9304 - 9309.
  • LATTOVA, E; BRYANT, J; SKRICKOVA, J; ZDRAHAL, Z; POPOVIC, M, 2016:Efficient Procedure for N-Glycan Analyses and Detection of Endo H-Like Activity in Human Tumor Specimens. JOURNAL OF PROTEOME RESEARCH 15 (8), p. 2777 - 2786.
  • SEDO, O; KORAN, M; JAKESOVA, M; MIKULIKOVA, R; BOHAC, M; ZDRAHAL, Z, 2016:Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry. FOOD CHEMISTRY 206 , p. 124 - 130.
  • SPONER, JE; SPONER, J; NOVAKOVA, O; BRABEC, V; SEDO, O; ZDRAHAL, Z; COSTANZO, G; PINO, S; SALADINO, R; DI MAURO, E, 2016:Emergence of the First Catalytic Oligonucleotides in a Formamide-Based Origin Scenario. CHEMISTRY-A EUROPEAN JOURNAL 22 (11), p. 3572 - 3586.

2015

  • DADAKOVA, K; HAVELKOVA, M; KURKOVA, B; TLOLKOVA, I; KASPAROVSKY, T; ZDRAHAL, Z; LOCHMAN, J, 2015:Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection. JOURNAL OF PROTEOMICS 119 , p. 143 - 153.
  • KARASEK, P; GRYM, J; ROTH, M; PLANETA, J; FORET, F, 2015:Etching of glass microchips with supercritical water. LAB ON A CHIP 15 (1), p. 311 - 318.
  • KOZAKOVA, L; VONDROVA, L; STEJSKAL, K; CHARALABOUS, P; KOLESAR, P; LEHMANN, AR; ULDRIJAN, S; SANDERSON, CM; ZDRAHAL, Z; PALECEK, JJ, 2015:The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex. CELL CYCLE 14 (6), p. 920 - 930.
  • KREJCI, J; STIXOVA, L; PAGACOVA, E; LEGARTOVA, S; KOZUBEK, S; LOCHMANOVA, G; ZDRAHAL, Z; SEHNALOVA, P; DABRAVOLSKI, S; HEJATKO, J; BARTOVA, E, 2015:Post-Translational Modifications of Histones in Human Sperm. JOURNAL OF CELLULAR BIOCHEMISTRY 116 (10), p. 2195 - 2209.
  • LATTOVA, E; BRABCOVA, A; BARTOVA, V; POTESIL, D; BARTA, J; ZDRAHAL, Z, 2015:N-Glycome Profiling of Patatins from Different Potato Species of Solanum Genus. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63 (12), p. 3243 - 3250.
  • NEMEC, A; KRIZOVA, L; MAIXNEROVA, M; SEDO, O; BRISSE, S; HIGGINS, PG, 2015:Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65 , p. 934 - 942.

2014

  • BALAZOVA, T; MAKOVCOVA, J; SEDO, O; SLANY, M; FALDYNA, M; ZDRAHAL, Z, 2014:The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling. FEMS MICROBIOLOGY LETTERS 353 (1), p. 77 - 84.
  • BALAZOVA, T; SEDO, O; STEFANIC, P; MANDIC-MULEC, I; VOS, M; ZDRAHAL, Z, 2014:Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion. RAPID COMMUNICATIONS IN MASS SPECTROMETRY 28 (17), p. 1855 - 1861.
  • BERNATIK, O; SEDOVA, K; SCHILLE, C; GANJI, RS; CERVENKA, I; TRANTIREK, L; SCHAMBONY, A; ZDRAHAL, Z; BRYJA, V, 2014:Functional Analysis of Dishevelled-3 Phosphorylation Identifies Distinct Mechanisms Driven by Casein Kinase 1 epsilon and Frizzled5. JOURNAL OF BIOLOGICAL CHEMISTRY 289 (34), p. 23520 - 23533.
  • DE GROOT, REA; GANJI, RS; BERNATIK, O; LLOYD-LEWIS, B; SEIPEL, K; SEDOVA, K; ZDRAHAL, Z; DHOPLE, VM; DALE, TC; KORSWAGEN, HC; BRYJA, V, 2014:Huwe1-Mediated Ubiquitylation of Dishevelled Defines a Negative Feedback Loop in the Wnt Signaling Pathway. SCIENCE SIGNALING 7 (317)
  • HAFIDH, S; POTESIL, D; FILA, J; FECIKOVA, J; CAPKOVA, V; ZDRAHAL, Z; HONYS, D, 2014:In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception. BIOCHEMICAL SOCIETY TRANSACTIONS 42 , p. 388 - 394.

2013

  • CINCAROVA, L; ZDRAHAL, Z; FAJKUS, J, 2013:New perspectives of valproic acid in clinical practice. EXPERT OPINION ON INVESTIGATIONAL DRUGS 22 (12), p. 1535 - 1547.
  • KONECNA, H; FRIDRICHOVA, D; LOCHMANOVA, G; BARTA, J; ZDRAHAL, Z, 2013:Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels. ELECTROPHORESIS 34 (13), p. 1969 - 1971.
  • SEDO, O; NEMEC, A; KRIZOVA, L; KACALOVA, M; ZDRAHAL, Z, 2013:Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. SYSTEMATIC AND APPLIED MICROBIOLOGY 36 (8), p. 572 - 578.
  • STEJSKAL, K; POTESIL, D; ZDRAHAL, Z, 2013:Suppression of Peptide Sample Losses in Autosampler Vials. JOURNAL OF PROTEOME RESEARCH 12 (6), p. 3057 - 3062.
  • ZD'ARSKA, M; ZATLOUKALOVA, P; BENITEZ, M; SEDO, O; POTESIL, D; NOVAK, O; SVACINOVA, J; PESEK, B; MALBECK, J; VASICKOVA, J; ZDRAHAL, Z; HEJATKO, J, 2013:Proteome Analysis in Arabidopsis Reveals Shoot- and Root-Specific Targets of Cytokinin Action and Differential Regulation of Hormonal Homeostasis. PLANT PHYSIOLOGY 161 (2), p. 918 - 930.

2012

  • BARTA, J; BARTOVA, V; ZDRAHAL, Z; SEDO, O, 2012:Cultivar Variability of Patatin Biochemical Characteristics: Table versus Processing Potatoes (Solanum tuberosum L.). JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60 (17), p. 4369 - 4378.
  • CINCAROVA, L; LOCHMANOVA, G; NOVAKOVA, K; SULTESOVA, P; KONECNA, H; FAJKUSOVA, L; FAJKUS, J; ZDRAHAL, Z, 2012:A combined approach for the study of histone deacetylase inhibitors. MOLECULAR BIOSYSTEMS 8 (11), p. 2937 - 2945.
  • DOKLADAL, L; OBORIL, M; STEJSKAL, K; ZDRAHAL, Z; PTACKOVA, N; CHALOUPKOVA, R; DAMBORSKY, J; KASPAROVSKY, T; JEANDROZ, S; ZD'ARSKA, M; LOCHMAN, J, 2012:Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. JOURNAL OF EXPERIMENTAL BOTANY 63 (5), p. 2203 - 2215.
  • KONECNA, H; MULLER, L; DOSOUDILOVA, H; POTESIL, D; BURSIKOVA, J; SEDO, O; MAROVA, I; ZDRAHAL, Z, 2012:Exploration of Beer Proteome Using OFFGEL Prefractionation in Combination with Two-Dimensional Gel Electrophoresis with Narrow pH Range Gradients. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60 (10), p. 2418 - 2426.
  • PEKAR, S; SMERDA, J; HRUSKOVA, M; SEDO, O; MUSTER, C; CARDOSO, P; ZDRAHAL, Z; KORENKO, S; BURES, P; LIZNAROVA, E; SENTENSKA, L, 2012:Prey-race drives differentiation of biotypes in ant-eating spiders. JOURNAL OF ANIMAL ECOLOGY 81 (4), p. 838 - 848.
  • SEDO, O; MAROVA, I; ZDRAHAL, Z, 2012:Beer fingerprinting by Matrix-Assisted Laser Desorption-Ionisation-Time of Flight Mass Spectrometry. FOOD CHEMISTRY 135 (2), p. 473 - 478.

2011

  • NOVAKOVA, K; SEDO, O; ZDRAHAL, Z, 2011:Mass Spectrometry Characterization of Plant Phosphoproteins. CURRENT PROTEIN & PEPTIDE SCIENCE 12 (2), p. 112 - 125.
  • SEDO, O; SEDLACEK, I; ZDRAHAL, Z, 2011:SAMPLE PREPARATION METHODS FOR MALDI-MS PROFILING OF BACTERIA. MASS SPECTROMETRY REVIEWS 30 (3), p. 417 - 434.
  • SEDO, O; VORAC, A; ZDRAHAL, Z, 2011:Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species. SYSTEMATIC AND APPLIED MICROBIOLOGY 34 (1), p. 30 - 34.

PROJEKTY

  • CIISB - České centrum pro integrativní strukturní biologii (LM2015043), MEYS, 2016 - 2019
  • CIISB4HEALTH - Česká infrastruktura pro integrativní strukturní biologii pro lidské zdraví (CZ.02.1.01/0.0/0.0/16_013/0001776), MEYS, 2017 - 2021
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Equipment Research group / CF Profile card
MALDI-TOF/TOF mass spectrometer CF: Proteomics Core Facility
nanoESI-Q-TOF MS CF: Proteomics Core Facility
Orbitrap Elite high resolution mass spectrometer CF: Proteomics Core Facility
Q-LIT mass spectrometer CF: Proteomics Core Facility
Vacuum concentrator CF: Proteomics Core Facility
Electrophoresis
LC-MALDI system LC-MALDI system
CF: Proteomics Core Facility
Medium-pressure chromatography modular system Medium-pressure chromatography modular system
CF: Proteomics Core Facility
Liquid Chromatography
Gel imaging system Gel imaging system
CF: Proteomics Core Facility
Isoelectric focusing system Isoelectric focusing system
CF: Proteomics Core Facility
Spot cutter Spot cutter
CF: Proteomics Core Facility
Vertical Gel Electrophoretic Chamber Vertical Gel Electrophoretic Chamber
CF: Proteomics Core Facility
Vertical Gel Electrophoretic Chamber Vertical Gel Electrophoretic Chamber
CF: Proteomics Core Facility
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