Změnit instituci
Pokročilé nano a mikrotechnologie Pokročilé materiály Strukturní biologie Gen. a prot. rostlin. systémů Molekulární medicína Výzkum mozku a lidské mysli Molekulární vet. medicína

Výzkumná skupina Helene Robert Boisivon

Vedoucí výzkumné skupiny
Researcher ID
Telefon: +420 54949 8421
E-mail:
Kancelář:

Research areas

  • Hormonal crosstalk for plant organogenesis
  • Lateral root formation as working model for cytokinin-auxin interaction
  • Influence of local auxin biosynthesis for seed and embryo development

Main objectives

  • Convergence of hormonal pathways on transport-dependent auxin distribution upstream of lateral root formation
  • Role of auxin-cytokinin interaction in lateral root formation
  • Unraveling the effect of local auxin production on seed and embryo development in Arabidopsis
  • Identification of components of hormonal crosstalk by genetic approaches
  • Formulation of general models for hormonal regulation of organogenesis

Content of research

Growth and development of plants are regulated by signaling substances such as plant hormones. In plants, interactions between hormonal pathways represent crucial factors that govern their action. The molecular basis for hormonal crosstalk is largely unknown. Our research aims to identify the molecular and cellular mechanism(s) underlying crosstalk of hormonal pathways in organogenesis and other plant developmental processes. We use two working models in Arabidopsis, lateral root formation and embryo development, as ideal experimental models to study mechanisms of plant hormone action, the molecular basis of their interactions, and the role of these interactions in organogenesis.

We aim to identify the key points in which auxin and other signaling pathways converge during lateral root formation and the molecular components involved in the process. Using cell type specific transcriptome analysis, we investigate the molecular events involved in auxin-cytokinin regulated lateral root organogenesis.

During embryogenesis, shoot and root primordia are specified at opposite extremities of the embryo. An embryonic local auxin production influences signaling pathways leading to the activation of developmental program for root and shoot specification. We investigate how auxin biosynthesis is regulated within the embryo.

In both models, we used mutant screens approaches to specifically target interactions between selected hormonal pathways. The acquired knowledge on molecular networks and their mutual interactions in lateral root organogenesis and embryo morphogenesis will be used to model these processes and to extrapolate them onto other developmental situations.

seznam / vizitky

Jméno a pozice

E-mail

Telefon

Helene Robert Boisivon, Ph.D.
Vedoucí výzkumné skupiny
+420 54949 8421
Souad Mroue, M.Sc.
Odborná pracovnice - PhD studentka
Mgr. Lenka Pátková
Laboratorní technik
Andrea Simeunovic, PhD
Odborná pracovnice ve výzkumu - postdoc
Mgr. Lucie Crhák Khaitová, Ph.D.
Odborná pracovnice ve výzkumu
+420 54949 8418

VYBRANÉ PUBLIKACE

2016

  • LIU, GC; GAO, S; TIAN, HY; WU, WW; ROBERT, HS; DING, ZJ, 2016:Local Transcriptional Control of YUCCA Regulates Auxin Promoted Root-Growth Inhibition in Response to Aluminium Stress in Arabidopsis. PLOS GENETICS 12 (10)
  • RAKUSOVA, H; ABBAS, M; HAN, HB; SONG, SY; ROBERT, HS; FRIML, J, 2016:Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity. CURRENT BIOLOGY 26 (22), p. 3026 - 3032.
  • VALUCHOVA, S; FULNECEK, J; PETROV, AP; TRIPSIANES, K; RIHA, K, 2016:A rapid method for detecting protein-nucleic acid interactions by protein induced fluorescence enhancement. SCIENTIFIC REPORTS 6
  • WATSON, JM; PLATZER, A; KAZDA, A; AKIMCHEVA, S; VALUCHOVA, S; NIZHYNSKA, V; NORDBORG, M; RIHA, K, 2016:Germline replications and somatic mutation accumulation are independent of vegetative life span in Arabidopsis. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113 (43), p. 12226 - 12231.

2015

  • ROBERT BOISIVON H; MROUE S; CRHAK KHAITOVA L; BENKOVA E, 2015:The importance of localized auxin production for morphogenesis of reproductive organs and embryos in Arabidopsis. Journal of Experimental Botany 66 (16), p. 5029 - 5042.
  • ROBERT, HS; GRUNEWALD, W; SAUER, M; CANNOOT, B; SORIANO, M; SWARUP, R; WEIJERS, D; BENNETT, M; BOUTILIER, K; FRIML, J, 2015:Plant embryogenesis requires AUX/LAX-mediated auxin influx. DEVELOPMENT 142 (4), p. 702 - 711.

2014

  • MARHAVY, P; DUCLERCQ, J; WELLER, B; FERARU, E; BIELACH, A; OFFRINGA, R; FRIML, J; SCHWECHHEIMER, C; MURPHY, A; BENKOVA, E, 2014:Cytokinin Controls Polarity of PIN1-Dependent Auxin Transport during Lateral Root Organogenesis. CURRENT BIOLOGY 24 (9), p. 1031 - 1037.
  • SMET, D; ZADNIKOVA, P; VANDENBUSSCHE, F; BENKOVA, E; VAN DER STRAETEN, D, 2014:Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids. NEW PHYTOLOGIST 202 (4), p. 1398 - 1411.

2013

  • CUESTA, C; WABNIK, K; BENKOVA, E, 2013:Systems approaches to study root architecture dynamics. FRONTIERS IN PLANT SCIENCE 4
  • PONTVIANNE, F; BLEVINS, T; CHANDRASEKHARA, C; MOZGOVA, I; HASSEL, C; PONTES, OMF; TUCKER, S; MOKROS, P; MUCHOVA, V; FAJKUS, J; PIKAARD, CS, 2013:Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states. GENES & DEVELOPMENT 27 (14), p. 1545 - 1550.
  • ROBERT, HS; GRONES, P; STEPANOVA, AN; ROBLES, LM; LOKERSE, AS; ALONSO, JM; WEIJERS, D; FRIML, J, 2013:Local Auxin Sources Orient the Apical-Basal Axis in Arabidopsis Embryos. CURRENT BIOLOGY 23 (24), p. 2506 - 2512.
  • WABNIK, K; ROBERT, HS; SMITH, RS; FRIML, J, 2013:Modeling Framework for the Establishment of the Apical-Basal Embryonic Axis in Plants. CURRENT BIOLOGY 23 (24), p. 2513 - 2518.

2012

  • VANSTRAELEN, M; BENKOVA, E, 2012:Hormonal Interactions in the Regulation of Plant Development. ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 28 28 , p. 463 - 487.

GRANTY

  • Unravelling the regulation of the local auxin production in Arabidopsis: a trigger for embryo patterning (3SGA5602), South Moravian Region - SoMoPro, 2014 - 2016
  • Molekulární mechanismy auxín-cytokinínové interakce v regulaci rostlinné organogenese (GA13-39982S), Czech Science Foundation - Standard Grants, 2013 - 2017

CURRENT RESEARCH INFRASTRUCTURE

  • Standard laboratory equipment for molecular biology, cell biology, plant hormone biology and plant cultivation under tightly regulated conditions (fytotrons).
  • Expertise and high-end instrumentation in advanced microscopy techniques, including high-end confocal microscopes (ZEISS 780 and ZEISS 700), light microscopes equipped with DIC objectives, ZEISS epifluorescence microscopes.
  • Immuno robot (Intavis), www.intavis.de/en/In_Situ_Detection/InsituPro_VSi/index.php

 

1. Molecular mechanisms of auxin biosynthesis regulation during embryo development in Arabidopsis

Supervisor: Hélene Robert Boisivon, Ph.D.

Annotation:

Introduction Food production mostly relies on a proper sexual plant reproduction. Therefore research in fundamental aspects of seed development is crucial to understand processes involved in seed viability. Embryo development follows a strict pattern of cell divisions and differentiations, which, if whenever affected, perturbs embryo shape and viability. The plant hormone auxin is a mediator of these developmental events. Dynamic changes in auxin distribution during embryo development are crucial for establishment of embryonic root and shoot. Previous work in the group showed that a local production of auxin regulates this embryonic auxin distribution. Our research projects focus on how environmental and endogenous signals are integrated at the cellular level into the regulation of this auxin transport both in embryonic and post-embryonic tissues. Project description The next challenge is to identify how this localized embryonic auxin production is regulated. This PhD project focuses on identification of novel regulators of auxin biosynthesis in embryos. A forward genetic screen combined with developmental and microscopic approaches in the model plant Arabidopsis thaliana will be employed to gain new insights into this developmental process. Techniques involved Genetics, Microscopy, Molecular Biology, Cellular Biology in context of plant reproduction. We are seeking an enthusiastic and motivated student who would be interested to carry out his/her PhD research project in our lab. The candidate should have MSc degree in the area of Life sciences. Experience in molecular biology/cell biology/plant culture/microscopy is a plus. English will be our communication language. We offer Ambitious laboratory facilities, young, international and dynamic work environment. Experience acquisition in a wide range of biological technics and methods. Experimental work on own project with the possibility of publication in top international journals.

References
ROBERT BOISIVON, H.;GRONES, P.;STEPANOVA, A.N.;ROBLES, L.M.;LOKERSE, A.S.;ALONSO, J.M.;WEIJERS, D.;FRIML, J. Local Auxin Sources Orient the Apical-Basal Axis in Arabidopsis Embryos. Current Biology, Cambridge: CELL PRESS, 2013, roč. 23, č. 24, s. 2506-2512. ISSN 0960-9822. doi:10.1016/j.cub.2013.09.039. ROBERT BOISIVON, H.;CRHÁK KHAITOVÁ, L.;MROUE, S.;FRIMLOVÁ, E. The importance of localized auxin production for morphogenesis of reproductive organs and embryos in Arabidopsis. Journal of Experimental Botany, Oxford: Oxford University Press, 2015, roč. 66, č. 16, s. 5029-5042. ISSN 0022-0957. doi:10.1093/jxb/erv256.

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