Growth and development of plants are regulated by signaling substances such as plant hormones. In plants, interactions between hormonal pathways represent crucial factors that govern their action. The molecular basis for hormonal crosstalk is largely unknown. Our research aims to identify the molecular and cellular mechanism(s) underlying crosstalk of hormonal pathways in organogenesis and other plant developmental processes. We use two working models in Arabidopsis, lateral root formation and embryo development, as ideal experimental models to study mechanisms of plant hormone action, the molecular basis of their interactions, and the role of these interactions in organogenesis.
We aim to identify the key points in which auxin and other signaling pathways converge during lateral root formation and the molecular components involved in the process. Using cell type specific transcriptome analysis, we investigate the molecular events involved in auxin-cytokinin regulated lateral root organogenesis.
During embryogenesis, shoot and root primordia are specified at opposite extremities of the embryo. An embryonic local auxin production influences signaling pathways leading to the activation of developmental program for root and shoot specification. We investigate how auxin biosynthesis is regulated within the embryo.
In both models, we used mutant screens approaches to specifically target interactions between selected hormonal pathways. The acquired knowledge on molecular networks and their mutual interactions in lateral root organogenesis and embryo morphogenesis will be used to model these processes and to extrapolate them onto other developmental situations.
seznam / vizitky
Jméno a pozice
|Helene Robert Boisivon, Ph.D.
Vedoucí výzkumné skupiny
|+420 54949 8421|
|Souad Mroue, M.Sc.
Odborná pracovnice - PhD studentka
|Mgr. Lenka Pátková
|Andrea Simeunovic, PhD
Odborná pracovnice ve výzkumu - postdoc
|Mgr. Lucie Crhák Khaitová, Ph.D.
Odborná pracovnice ve výzkumu
|+420 54949 8418|
Supervisor: Hélene Robert Boisivon, Ph.D.
Introduction Food production mostly relies on a proper sexual plant reproduction. Therefore research in fundamental aspects of seed development is crucial to understand processes involved in seed viability. Embryo development follows a strict pattern of cell divisions and differentiations, which, if whenever affected, perturbs embryo shape and viability. The plant hormone auxin is a mediator of these developmental events. Dynamic changes in auxin distribution during embryo development are crucial for establishment of embryonic root and shoot. Previous work in the group showed that a local production of auxin regulates this embryonic auxin distribution. Our research projects focus on how environmental and endogenous signals are integrated at the cellular level into the regulation of this auxin transport both in embryonic and post-embryonic tissues. Project description The next challenge is to identify how this localized embryonic auxin production is regulated. This PhD project focuses on identification of novel regulators of auxin biosynthesis in embryos. A forward genetic screen combined with developmental and microscopic approaches in the model plant Arabidopsis thaliana will be employed to gain new insights into this developmental process. Techniques involved Genetics, Microscopy, Molecular Biology, Cellular Biology in context of plant reproduction. We are seeking an enthusiastic and motivated student who would be interested to carry out his/her PhD research project in our lab. The candidate should have MSc degree in the area of Life sciences. Experience in molecular biology/cell biology/plant culture/microscopy is a plus. English will be our communication language. We offer Ambitious laboratory facilities, young, international and dynamic work environment. Experience acquisition in a wide range of biological technics and methods. Experimental work on own project with the possibility of publication in top international journals.
ROBERT BOISIVON, H.;GRONES, P.;STEPANOVA, A.N.;ROBLES, L.M.;LOKERSE, A.S.;ALONSO, J.M.;WEIJERS, D.;FRIML, J. Local Auxin Sources Orient the Apical-Basal Axis in Arabidopsis Embryos. Current Biology, Cambridge: CELL PRESS, 2013, roč. 23, č. 24, s. 2506-2512. ISSN 0960-9822. doi:10.1016/j.cub.2013.09.039. ROBERT BOISIVON, H.;CRHÁK KHAITOVÁ, L.;MROUE, S.;FRIMLOVÁ, E. The importance of localized auxin production for morphogenesis of reproductive organs and embryos in Arabidopsis. Journal of Experimental Botany, Oxford: Oxford University Press, 2015, roč. 66, č. 16, s. 5029-5042. ISSN 0022-0957. doi:10.1093/jxb/erv256.
19. září 2017 14:37
WHEN: November 14-16, 2017 WHERE: CF MAFIL, CEITEC MU, University Campus Bohunice, Brno
19. září 2017 9:15
Markéta Tesařová je sice teprve v magisterském studiu oboru Fyzikální inženýrství a nanotechnologie FSI VUT, ale už sbírá úspěchy i v oblasti…