Změnit instituci
Pokročilé nano a mikrotechnologie Pokročilé materiály Strukturní biologie Gen. a prot. rostlin. systémů Molekulární medicína Výzkum mozku a lidské mysli Molekulární vet. medicína

Výzkumná skupina Jana Hejátka

Vedoucí výzkumné skupiny
Researcher ID
Telefon: +420 54949 4165
E-mail: ,
Kancelář:

Research areas

  • Role of cytokinins in the vascular tissue and root apical meristem formation and developmentczech bio logo nove
  • Interaction of cytokinin and other hormones, particularly auxin
  • Interaction of cytokinins with light
  • Structural basis of multistep phosphorelay signaling in plants
  • Plant proteome and phosphoproteome response to cytokinins
  • Employing the knowledge of molecular mechanisms of multistep phosphorelay-based signalling in molecular breeding
  • Production of bioactive compounds in plant production systems

Main objectives

Determination of molecular mechanisms governing hormonal regulations and their functions in plant development.

Content of research

The research group is interested in the hormonal regulation of plant development and underlying molecular mechanisms with particular emphasis on the understanding of cytokinin signalling, action and interaction with other plant growth regulators.

Plant cells are well known for their tremendous developmental plasticity. Plant hormones, particularly auxins and cytokinins were found to be major regulators of intrinsic developmental programs associated with changes of differentiation status of plant cells and tissues. That allows de novo formation of entire plants from virtually all types of specialised plant tissues. Identification of basic molecular principles involved in the regulation of plant cell division and differentiation will provide developmental model useful in the comparative biology approaches and identification of corresponding regulatory and developmental events in animal and particularly human cell systems.

In the group of Functional Genomics and Proteomics of Plants, we are interested in the molecular mechanisms underlying the regulation of plant development by plant hormones cytokinins (CKs) and their interaction with other plant hormones, e.g. auxin. In our studies we employ comprehensive approaches including forward and reverse genetics, proteomics, protein biochemistry, protein structure analysis and bioinformatics to recognise the principles of complex molecular events involved in the cytokinin signal transduction and action.

We are particularly interested in study of following problems:

  1. Interaction of auxin and cytokinins in the processes of de novo organogenesis, regulation of root meristem patterning and vascular tissue development. We are interested in the identification of molecular targets acting downstream the CK signalling pathways and the role of gene regulatory networks constituting CKdependent developmental circuits.
  2. Elucidating molecular determinants of specifi city in multistep phosphorelay (MSP), with special emphasis on the role of MSP in CK signalling. Particularly we are interested in the structural analysis of intracellular receiver domains of sensory histidine kinases that, as we have found, specifically interact with downstream signaling His-containing phosphotransfer proteins, determining thus specificity in plant MSP pathways.
  3. Hormonal regulations of plant proteome, particularly the study of CK-dependent proteome changes with the aim of identifying novel regulatory targets of CK-mediated regulations of plant development and elucidating molecular mechanisms underlying interaction of CK with other plant growth regulators, e.g. ethylene.
  4. Development and application of novel approaches, e.g. immunomodulation (production of scFv fragments recognising both cytokinins and CK signalling proteins in vivo) to modulate CK-dependent regulation of plant development.

Extending our fundamental research and use of its results in applied science and development of novel strategies useful in e.g. molecular breeding or using of plant systems for biotherapeutics production.

seznam / vizitky

Jméno a pozice

E-mail

Telefon

doc. RNDr. Jan Hejátko, Ph.D.
Vedoucí výzkumné skupiny
+420 54949 4165
Ing. Blanka Pekárová, Ph.D.
Výzkumný pracovník
+420 54949 3029
Mgr. Markéta Žďárská, Ph.D.
Výzkumný pracovník - postdoc
+420 54949 3553
Mónika Hrtyan
Laboratorní technička
Romana Hejátková
Laborant
Ing. Šárka Michlíčková
Laborantka
+420 54949 5410
Bc. Martina Válková
Laborant
+420 54949 7904
Mgr. Ivana Urbánková
Odborný pracovník
Ivan Kashkan
Mgr. Jakub Zeman
Manažer výzkumných projektů
+420 54949 6795
Mgr. Zuzana Gelová
PhD student
Agnieszka Szmitkowska, M.Sc.
Candelas Maria Paniagua Correas, Dr.
Tomáš Faltus
Amel Yamoune, M.Sc.
Elena Zemlyanskaya, M.Sc.
Mgr. Kamil Růžička, Dr. rer. nat.
Výzkumný pracovník
Aswathy Jayasree, Ph.D.
Veronika Balakhonova
Mgr. Pavel Veverka
PhD student
Kseniya Timofeyenko
Abigail Rubiato Cuyacot
Bc. Tomáš Konečný
Bc. Michal Ondruš
Bc. Petra Zvolánková
Anna Alaxinová
PhD student
Bc. Veronika Bilanovičová
Mgr. Anna Bílková
Siarhei Dabravolski
PhD student
Petra Elblová
Eliška Špačková
Mgr. Markéta Šámalová, Ph.D.
+420 54949 8476
Bc. Gabriela Jalová
Organizační referent
+420 54949 1454

VYBRANÉ PUBLIKACE

2017

  • DOBISOVA, T; HRDINOVA, V; CUESTA, C; MICHLICKOVA, S; URBANKOVA, I; HEJATKOVA, R; ZADNIKOVA, P; PERNISOVA, M; BENKOVA, E; HEJATKO, J, 2017:Light Controls Cytokinin Signaling via Transcriptional Regulation of Constitutively Active Sensor Histidine Kinase CKI1. PLANT PHYSIOLOGY 174 (1), p. 387 - 404.
  • DOBISOVA, T; HRDINOVA, V; CUESTA, C; MICHLICKOVA, S; URBANKOVA, I; HEJATKOVA, R; ZADNIKOVA, P; PERNISOVA, M; BENKOVA, P; HEJATKO, J, 2017:Light-regulated expression of sensor histidine kinase CKI1 controls cytokinin-related development. PLANT PHYSIOLOGY

2016

  • NODZYNSKI, T; VANNESTE, S; ZWIEWKA, M; PERNISOVA, M; HEJATKO, J; FRIML, J, 2016:Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components. MOLECULAR PLANT 9 (11), p. 1504 - 1519.
  • PEKAROVA, B; SZMITKOWSKA, A; DOPITOVA, R; DEGTJARIK, O; ZIDEK, L; HEJATKO, J, 2016:Structural Aspects of Multistep Phosphorelay-Mediated Signaling in Plants. MOLECULAR PLANT 9 (1), p. 71 - 85.
  • PERNISOVA, M; PRAT, T; GRONES, P; HARUSTIAKOVA, D; MATONOHOVA, M; SPICHAL, L; NODZYNSKI, T; FRIML, J; HEJATKO, J, 2016:Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis. NEW PHYTOLOGIST 212 (2), p. 497 - 509.

2015

  • DIDI, V; JACKSON, P; HEJATKO, J, 2015:Hormonal regulation of secondary cell wall formation. JOURNAL OF EXPERIMENTAL BOTANY 66 (16), p. 5015 - 5027.
  • HRTYAN, M; SLIKOVA, E; HEJATKO, J; RUZICKA, K, 2015:RNA processing in auxin and cytokinin pathways. JOURNAL OF EXPERIMENTAL BOTANY 66 (16), p. 4897 - 4912.
  • JUPA, R; DIDI, V; HEJATKO, J; GLOSER, V, 2015:An improved method for the visualization of conductive vessels in Arabidopsis thaliana inflorescence stems. FRONTIERS IN PLANT SCIENCE 6
  • KREJCI, J; STIXOVA, L; PAGACOVA, E; LEGARTOVA, S; KOZUBEK, S; LOCHMANOVA, G; ZDRAHAL, Z; SEHNALOVA, P; DABRAVOLSKI, S; HEJATKO, J; BARTOVA, E, 2015:Post-Translational Modifications of Histones in Human Sperm. JOURNAL OF CELLULAR BIOCHEMISTRY 116 (10), p. 2195 - 2209.
  • KUDEROVA, A; GALLOVA, L; KURICOVA, K; NEJEDLA, E; CURDOVA, A; MICENKOVA, L; PLIHAL, O; SMAJS, D; SPICHAL, L; HEJATKO, J, 2015:Identification of AHK2-and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues. JOURNAL OF EXPERIMENTAL BOTANY 66 (1), p. 339 - 353.
  • RUZICKA, K; URSACHE, R; HEJATKO, J; HELARIUTTA, Y, 2015:Xylem development - from the cradle to the grave. NEW PHYTOLOGIST 207 (3), p. 519 - 535.
  • ZDARSKA, M; DOBISOVA, T; GELOVA, Z; PERNISOVA, M; DABRAVOLSKI, S; HEJATKO, J, 2015:Illuminating light, cytokinin, and ethylene signalling crosstalk in plant development. JOURNAL OF EXPERIMENTAL BOTANY 66 (16), p. 4913 - 4931.

2014

  • BORKOVCOVA, P; PEKAROVA, B; VALKOVA, M; DOPITOVA, R; BRZOBOHATY, B; JANDA, L; HEJATKO, J, 2014:Antibodies against CKI1(RD), a receiver domain of the sensor histidine kinase in Arabidopsis thaliana: From antigen preparation to in planta immunolocalization. PHYTOCHEMISTRY 100 , p. 6 - 15.
  • ZABRADY, M; HRDINOVA, V; MULLER, B; CONRAD, U; HEJATKO, J; JANDA, L, 2014:Targeted In Vivo Inhibition of Specific Protein-Protein Interactions Using Recombinant Antibodies. PLOS ONE 9 (10)

2013

  • BENITEZ, M; HEJATKO, J, 2013:Dynamics of Cell-Fate Determination and Patterning in the Vascular Bundles of Arabidopsis thaliana. PLOS ONE 8 (5)
  • NOVACEK, J; JANDA, L; DOPITOVA, R; ZIDEK, L; SKLENAR, V, 2013:Efficient protocol for backbone and side-chain assignments of large, intrinsically disordered proteins: transient secondary structure analysis of 49.2 kDa microtubule associated protein 2c. JOURNAL OF BIOMOLECULAR NMR 56 (4), p. 291 - 301.
  • ZD'ARSKA, M; ZATLOUKALOVA, P; BENITEZ, M; SEDO, O; POTESIL, D; NOVAK, O; SVACINOVA, J; PESEK, B; MALBECK, J; VASICKOVA, J; ZDRAHAL, Z; HEJATKO, J, 2013:Proteome Analysis in Arabidopsis Reveals Shoot- and Root-Specific Targets of Cytokinin Action and Differential Regulation of Hormonal Homeostasis. PLANT PHYSIOLOGY 161 (2), p. 918 - 930.

2012

  • DOKLADAL, L; OBORIL, M; STEJSKAL, K; ZDRAHAL, Z; PTACKOVA, N; CHALOUPKOVA, R; DAMBORSKY, J; KASPAROVSKY, T; JEANDROZ, S; ZD'ARSKA, M; LOCHMAN, J, 2012:Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. JOURNAL OF EXPERIMENTAL BOTANY 63 (5), p. 2203 - 2215.

2011

  • PEKAROVA, B; KLUMPLER, T; TRISKOVA, O; HORAK, J; JANSEN, S; DOPITOVA, R; BORKOVCOVA, P; PAPOUSKOVA, V; NEJEDLA, E; SKLENAR, V; MAREK, J; ZIDEK, L; HEJATKO, J; JANDA, L, 2011:Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. PLANT JOURNAL 67 (5), p. 827 - 839.

před rokem 2011

  • HEJATKO, J; RYU, H; KIM, GT; DOBESOVA, R; CHOI, S; CHOI, SM; SOUCEK, P; HORAK, J; PEKAROVA, B; PALME, K; BRZOBOHATY, B; HWANG, I, 2009: The histidine kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 regulate vascular tissue development in Arabidopsis shoots. PLANT CELL 21 (7), p. 2008 - 2021.
  • PERNISOVA, M; KLIMA, P; HORAK, J; VALKOVA, M; MALBECK, J; SPUCEK, P; REICHMAN, P; HOYEROVA, K; DUBOVA, J; FRIML, J; ZAZIMALOVA, E; HEJATKO, J, 2009: Cytokinins modulate auxin-induced organogenesis in plants via regulation of the auxin efflux. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE U.S.A. 106, p. 3609 - 3614.
  • HEJATKO, J; BLILOU, I; BREWER, PB; FRIML, J; SCHERES, B; BENKOVA, E, 2006: In situ hybridisation technique for mRNA detection in whole mount Arabidopsis samples. NATURE PROTOCOLS 1, p. 1939 - 1946.
  • HEJATKO, J; PERNISOVA, M; ENEVA, T; PALME, K; Brzobohatý, B, 2003: The putative sensor histidine kinase CKI1 is involved in female gametophyte development in Arabidopsis. MOLECULAR GENETICS AND GENOMICS 269, p. 443 - 453.


PATENTY

  • HEJATKO, J; DOBESOVA, R; DUBOVA. J; HWANG, I; RYU, H, 2009: Method of regulation of biomass production in plants. Czech patent No. 300145, Granted on January 15, 2009.  Applicants: Masaryk University and POSTECH Academy Industry Foundation

GRANTY

  • Dešifrování molekulárních mechanizmů integrace světelné a hormonální regulace vývoje rostlin (GA15-22000S), Czech Science Foundation - Standard Grants, 2015 - 2017
  • Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (GAP305/11/0756), Czech Science Foundation - Standard Grants, 2011 - 2014
  • Význam interakcí cytokininů a auxinu v regulaci gravitropismu kořene u Arabidopsis thaliana. (GAP501/11/1150), Czech Science Foundation - Standard Grants, 2011 - 2013
  • Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana (GA521/09/1699), Czech Science Foundation - Standard Grants, 2009 - 2012
  • Identification of molecular components and the mechanism of polar targeting of PIN auxin transport proteins in Arabidopsis thaliana (IAA601630703), Academy of Sciences of the Czech Republic - Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Rep., 2007 - 2011
  • Regulation of morphogenesis of plant cells and organs (LC06034), MEYS - Basic Research Centres, 2006 - 2011

CURRENT RESEARCH INFRASTRUCTURE

Equipment for cultivation of plants under tightly regulated conditions (fytotrons) and under defined light quality and quantity. Expertise and high-end instrumentation in advanced microscopy techniques that include high-end confocal microscope equipped with white laser, highly sensitive hybrid detectors and module for fluorescence life-time imaging (FLIM) and fluorescence correlation spectroscopy (FCS). Image analysis software allowing highly sensitive and specific fluorescence imaging in living cells via fluorescence intensity decay shape analysis microscopy (FIDSAM). High-throughput system for automated microscopy (Olympus “.slide”) and horizontal confocal macroscope (modified Nikon AZ-C1 system), allowing detailed, real-time fluorescent protein localisation and dynamics in vivo.

Video of High-throughput system for automated microscopy (Olympus “.slide”) can be found HERE.

supplement-figure-1

1. Molecular mechanisms underlying hormonal regulation of cell wall modifications in Arabidopsis

Supervisor: doc. Mgr.Jan Hejátko, Ph.D.
Consultants: Philip Anthony P. Jackson, Ph.D., Willi Sascha Riber, Dr. rer. nat.  

Annotation:

We are interested in the study of plant hormonal signaling pathways, particularly cytokinins in the development of model plant Arabidopsis thaliana1-3. Biomechanical properties of cell wall were shown to have strong impact on plant organogenesis 4,5. Recently we have identified several potential targets mediating the cytokinin control over the cell wall properties. The goal of the thesis will be to describe the role of cytokinin-driven regulation of the cell wall composition in plant development. The project will include molecular biology approaches, transgenic and mutant lines preparation, advanced histological techniques (e.g. in situ immunolocalization) and confocal microscopy imaging.

  • We expect: Highly motivated candidates with ambition of reaching fundamental findings that will change our view on principles guiding plant development.
  • We offer: Experimental work on own project with the possibility of publication in top-ranked international journals, nice international lab staff, world-class lab equipment, attractive environment of novel university campus, help with both intellectual and practical problems, competitive salary corresponding to the work efficiency, abroad working stays in frame of the project.

References

  • 1. Pernisova, M. et al. Cytokinins modulate auxin-induced organogenesis in plants via regulation of the auxin efflux. Proc Natl Acad Sci U S A 106, 3609-14 (2009).
  • 2. Pekarova, B. et al. Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. Plant J 67, 827-39 (2011).
  • 3. Hejatko, J. et al. The Histidine Kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 Regulate Vascular Tissue Development in Arabidopsis Shoots. Plant Cell 21, 2008-21 (2009).
  • 4. Fleming, A.J., McQueenMason, S., Mandel, T. & Kuhlemeier, C. Induction of leaf primordia by the cell wall protein expansion. Science 276, 1415-1418 (1997).
  • 5. Peaucelle, A. et al. Pectin-induced changes in cell wall mechanics underlie organ initiation in Arabidopsis. Current biology : CB 21, 1720-6 (2011).

2. Probing the role of plant Ser/Thr kinases and phosphorylation events in the development of the cytokinin response

Supervisor: Philip Anthony P. Jackson, Ph.D.
Consultant: doc. RNDr. Jan Hejátko, Ph.D.

Annotation:

Cytokinins are recognised to play a central and profound role in plant development and in the last decade, some of the molecular components involved in CK signal transduction and feed-back regulatory mechanisms been elucidated. However, further unknown components, including those involved in early, transcriptionally independent or down-stream modulatory mechanisms are expected to play important roles in the shaping of the cytokinin response. Recent work has shown that exogenous CK effects substantial, largely non-transcriptional changes of the plant proteome. More specifically, CK induces rapid (less or equal 15 min) changes in the phosphoproteome, including CK-regulated phosphatases, MAPKs and CIPKs suggesting that early CK-mediated alterations to Ser/Thr phosphorylation events and cascades is an essential additional route by which CK signals are transduced to further modulate the CK-response. The goals of the project would be to use advanced genetics, phenotyping, molecular biology and proteomic approaches in Arabidopsis to i) confirm a role for selected proteins active in phosphorylation events in the development of the CK response and ii) identify additional up- and down-stream components of this novel pathway. We expect: Highly motivated candidates with ambition of reaching top-ranked results. We offer: Experimental work on own project with the possibility of publication in distinguished international journals, excellent lab staff, world-class lab equipment including one of the best recently available confocal microscopes, LC-MS/MS, attractive environment of novel university campus, help with both intellectual and practical problems, competitive salary corresponding to the work efficiency.

3. Role of upstream open reading frames in auxin dependent processes

Supervisor: Mgr. Kamil Růžička, Dr. rer. nat.
Consultants: doc. RNDr. Jan Hejátko, Ph.D.

Annotation:

Upstream open reading frames (uORFs) are important post transcriptional regulatory elements typically situated in the gene 5’-untranslated regions. They are proposed to regulate (repress) expression of their downstream main ORFs. However, despite their large occurrence in significant part of eukaryotic (and plant) genes, their functional relevance has been poorly studied up to now. In addition, the role of auxin in uORF mediate signaling has been also suggested. To resolve this, we set up a bioinformatics pipeline to find uORFs in auxin-related genes. We took advantage of numerous sequenced plant genomes and determined conservation as a measure of expected functional relevance of a putative uORF found. Using sequence conservation as a hint again, we further aim to expand our bioinformatics pipeline to identify also conserved noncanonical (non-AUG initiated) uORFs to get more precise list of uORFs present in genes directly connected with auxin dependent processes. In addition, we already confirmed the presence of a highly conserved uORF in the gene encoding crucial transcriptional regulator AUXIN RESPONSE FACTOR 4 (ARF4). In the experimental part of the project, it will be tested a functional impact of this uORF on the expression the ARF4 main ORF and the resulting functional and phenotypic consequences.

4. The role of cytokinins in regulatory mechanisms of auxin induced de novo organogenesis

Supervisor: Mgr. Markéta Pernisová, Ph.D.
Consultant: doc. RNDr. Jan Hejátko, Ph.D.

Annotation:

Our lab is focused on plant hormonal signaling pathways, particularly cytokinins in the development of model plant Arabidopsis thaliana. Organogenesis is induced by other phytohormone – auxin, and identity of newly formed organs depends on auxin/cytokinin concentration ratio. In auxin only roots are formed. In high cytokinin/auxin ratio shoots are formed. Thus cytokinins are able to modulated auxin induced organogenesis, but regulatory mechanisms are still poorly known. The goal of the project will be i) to analyze cytokinin effect in de novo organogenesis and ii) to identify and analyze regulatory factors important for that process. We expect: creativity, self-reliance, willingness to learn new things. We offer: experimental work on own project, friendly lab staff, excellent lab equipment, high-end confocal microscopes, attractive environment of novel university campus, help with both intellectual and practical problems, competitive salary corresponding to the work efficiency.

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