Změnit instituci
Pokročilé nano a mikrotechnologie Pokročilé materiály Strukturní biologie Gen. a prot. rostlin. systémů Molekulární medicína Výzkum mozku a lidské mysli Molekulární vet. medicína

Výzkumná skupina Jiřího Fajkuse

Vedoucí výzkumné skupiny
Researcher ID
Telefon: +420 54949 4003
E-mail: ,
Kancelář:
Zástupce vedoucího výzkumné skupiny
Researcher ID
Telefon: +420 54949 8063
E-mail: ,
Kancelář:

Research areas

  • Structure, evolution and maintenance of telomeres and their roles in chromosome stability and plant speciation; this includes the characterization of nucleoprotein composition of telomeres and telomerases, biophysical analysis of interactions between telomere components by quantitative methods, analysis of structure-function relationships of telomerase subdomains and analysis of alternative (telomerase independent) strategies of telomere maintenance
  • Epigenetic mechanisms in the regulation of gene expression, chromosome stability and telomere homeostasis
  • Structure, evolution and functions of SMC complexes; the characterization of SMC5-6 complex subunits and MAGE proteins in vitro and in vivo; their roles in DNA repair and chromosome dynamics

Main objectives

  • To investigate epigenetic processes involved in regulation of telomere maintenance
  • To clarify connections between dynamics of telomere composition and DNA damage response
  • To explore evolution of telomeres, telomerase and alternative mechanisms of telomere maintenance
  • To decipher functional links between chromatin (nucleosome) assembly and genome stability
  • To examine interactions and functions of  SMC5-6 complex subunits and MAGE proteins
     

Content of research

Chromatin is a supramolecular complex of DNA, proteins and other associated molecules (e.g. RNA species). It is the building material of chromosomes, which can be observed during cell division in their most condensed state. Chromatin was first discovered in plant cells – as were the cells themselves, or genes.

Since eukaryotic genomes are folded into chromatin, all genome functions occur in the context of this highly dynamic structure. Understanding processes such as DNA replication and repair, transcription or cell differentiation thus requires understanding structure and function of chromatin, and its specific domains like centromeres, telomeres or nucleoli.

While the nucleotide sequence of the DNA component of chromatin constitutes the genetic material of the cell, the other chromatin components (and also modifications of bases in DNA itself) participate in so-called epigenetic functions. These include spatiotemporal regulation of gene activity and DNA replication, correct and precise segregation of genetic material to daughter cells, maintenance of chromosome stability, and protection of genetic material from damage. Importantly, the chromatin structure compacts several metres of genomic DNA to fit the size of a cell nucleus (several microns in diameter) while keeping it functional despite the high degree of compaction (105-106 fold).

Our research group integrates studies in the field of telomere biology, chromatin structure and epigenetics. Using unique features of plant systems (namely their high developmental plasticity), and their comparison to yeast or animal models, we aim to characterise pathways involved in control of chromosome stability and distinguish between specific and general mechanisms involved. Outcomes of our studies (e.g., understanding mechanisms contributing to genome stability, aging or adaptation to changing environmental conditions) can be applied in agriculture, biotechnologies or medicine.

seznam / vizitky

Jméno a pozice

E-mail

Telefon

prof. RNDr. Jiří Fajkus, CSc.
Vedoucí výzkumné skupiny
+420 54949 4003
doc. Mgr. Jan Paleček, Dr. rer. nat.
Vedoucí výzkumný pracovník
+420 54949 6128
doc. Mgr. Miloslava Fojtová, CSc.
Výzkumný pracovník
+420 54949 8063
doc. Mgr. Ctirad Hofr, Ph.D.
Výzkumný pracovník
+420 54949 5952
RNDr. Dagmar Zachová
Výzkumný pracovník
+420 54949 5601
Mgr. Martina Dvořáčková, Ph.D.
Výzkumný pracovník - postdoc
+420 54949 5403
Mgr. Kateřina Havlová
PhD student
+420 54949 5354
Mgr. Ivona Nečasová
PhD student
+420 54949 8155
Mgr. Lucie Vondrová
PhD student
+420 54949 5863
Lucie Němcová
Referent
+420 54949 4482
Marcela Šefčíková
Technik
+420 54949 6351
Monika Chropovská
+420 54949 2682
Mgr. Konstantin Kutashev
+420 54949 8139
Mgr. Martin Stojaspal
Student
Mgr. Pavel Veverka
PhD student
Mgr. Martina Nešpor Dadejová, Ph.D.
+420 54949 2655
Mgr. Tomáš Crhák
PhD student
+420 54949 2655
Mgr. Lenka Jurčišinová
PhD student
+420 54949 5863
Ing. Tomáš Janovič
Jana Kapustová
Technik
+420 54949 1494
Mgr. Markéta Pernisová, Ph.D.
Výzkumný pracovník
+420 54949 6470
Mgr. Marek Adamus
PhD student
Mgr. Kateřina Jůzová
PhD student
+420 54949 5863
Mgr. Edita Balkóová
+420 54949 5863
Mgr. Petr Fajkus
PhD student
+420 54949 2655
Mgr. Martin Lyčka
Tomáš Faltus

VYBRANÉ PUBLIKACE

2017

  • DOBISOVA, T; HRDINOVA, V; CUESTA, C; MICHLICKOVA, S; URBANKOVA, I; HEJATKOVA, R; ZADNIKOVA, P; PERNISOVA, M; BENKOVA, E; HEJATKO, J, 2017:Light Controls Cytokinin Signaling via Transcriptional Regulation of Constitutively Active Sensor Histidine Kinase CKI1. PLANT PHYSIOLOGY 174 (1), p. 387 - 404.
  • DOBISOVA, T; HRDINOVA, V; CUESTA, C; MICHLICKOVA, S; URBANKOVA, I; HEJATKOVA, R; ZADNIKOVA, P; PERNISOVA, M; BENKOVA, P; HEJATKO, J, 2017:Light-regulated expression of sensor histidine kinase CKI1 controls cytokinin-related development. PLANT PHYSIOLOGY
  • MAJERSKA, J; SCHRUMPFOVA, PP; DOKLADAL, L; SCHOROVA, S; STEJSKAL, K; OBORIL, M; HONYS, D; KOZAKOVA, L; POLANSKA, PS; SYKOROVA, E, 2017:Tandem affinity purification of AtTERT reveals putative interaction partners of plant telomerase in vivo. PROTOPLASMA 254 (4), p. 1547 - 1562.
  • VALUCHOVA, S; FULNECEK, J; PROKOP, Z; STOLT-BERGNER, P; JANOUSKOVA, E; HOFR, C; RIHA, K, 2017:Protection of Arabidopsis Blunt-Ended Telomeres Is Mediated by a Physical Association with the Ku Heterodimer. PLANT CELL 29 (6), p. 1533 - 1545.

2016

  • FAJKUS, P; PESKA, V; SITOVA, Z; FULNECKOVA, J; DVORACKOVA, M; GOGELA, R; SYKOROVA, E; HAPALA, J; FAJKUS, J, 2016:Allium telomeres unmasked: the unusual telomeric sequence (CTCGGTTATGGG)(n) is synthesized by telomerase. PLANT JOURNAL 85 (3), p. 337 - 347.
  • HAVLOVA, K; DVORACKOVA, M; PEIRO, R; ABIA, D; MOZGOVA, I; VANSACOVA, L; GUTIERREZ, C; FAJKUS, J, 2016:Variation of 45S rDNA intergenic spacers in Arabidopsis thaliana. PLANT MOLECULAR BIOLOGY 92 (2016-05-04T00:00:00.000), p. 457 - 471.
  • NODZYNSKI, T; VANNESTE, S; ZWIEWKA, M; PERNISOVA, M; HEJATKO, J; FRIML, J, 2016:Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components. MOLECULAR PLANT 9 (11), p. 1504 - 1519.
  • PAVLISTOVA, V; DVORACKOVA, M; JEZ, M; MOZGOVA, I; MOKROS, P; FAJKUS, J, 2016:Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes. PLANT JOURNAL 88 (3), p. 411 - 424.
  • PERNISOVA, M; PRAT, T; GRONES, P; HARUSTIAKOVA, D; MATONOHOVA, M; SPICHAL, L; NODZYNSKI, T; FRIML, J; HEJATKO, J, 2016:Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis. NEW PHYTOLOGIST 212 (2), p. 497 - 509.
  • SEPSIOVA, R; NECASOVA, I; WILLCOX, S; PROCHAZKOVA, K; GORILAK, P; NOSEK, J; HOFR, C; GRIFFITH, JD; TOMASKA, L, 2016:Evolution of Telomeres in Schizosaccharomyces pombe and Its Possible Relationship to the Diversification of Telomere Binding Proteins. PLOS ONE 11 (4)
  • SCHRUMPFOVA, PP; VYCHODILOVA, I; HAPALA, J; SCHOROVA, S; DVORACEK, V; FAJKUS, J, 2016:Telomere binding protein TRB1 is associated with promoters of translation machinery genes in vivo. PLANT MOLECULAR BIOLOGY 90 (2016-02-01T00:00:00.000), p. 189 - 206.
  • VAN DER CRABBEN, SN; HENNUS, MP; MCGREGOR, GA; RITTER, DI; NAGAMANI, SCS; WELLS, OS; HARAKALOVA, M; CHINN, IK; ALT, A; VONDROVA, L; HOCHSTENBACH, R; VAN MONTFRANS, JM; TERHEGGEN-LAGRO, SW; VAN LIESHOUT, S; VAN ROOSMALEN, MJ; RENKENS, I; DURAN, K; NIJMAN, IJ; KLOOSTERMAN, WP; HENNEKAM, E; ORANGE, JS; VAN HASSELT, PM; WHEELER, DA; PALECEK, JJ; LEHMANN, AR; OLIVER, AW; PEARL, LH; PLON, SE; MURRAY, JM; VAN HAAFTEN, G, 2016:Destabilized SMC5/6 complex leads to chromosome breakage syndrome with severe lung disease. JOURNAL OF CLINICAL INVESTIGATION 126 (8), p. 2881 - 2892.
  • ZABRADY, K; ADAMUS, M; VONDROVA, L; LIAO, C; SKOUPILOVA, H; NOVAKOVA, M; JURCISINOVA, L; ALT, A; OLIVER, AW; LEHMANN, AR; PALECEK, JJ, 2016:Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. NUCLEIC ACIDS RESEARCH 44 (3), p. 1064 - 1079.

2015

  • DOKLADAL, L; HONYS, D; RANA, R; LEE, LY; GELVIN, SB; SYKOROVA, E, 2015:cDNA Library Screening Identifies Protein Interactors Potentially Involved in Non-Telomeric Roles of Arabidopsis Telomerase. FRONTIERS IN PLANT SCIENCE 6
  • DVORACKOVA, M; FOJTOVA, M; FAJKUS, J, 2015:Chromatin dynamics of plant telomeres and ribosomal genes. PLANT JOURNAL 83 (1), p. 18 - 37.
  • FOJTOVA, M; SYKOROVA, E; NAJDEKROVA, L; POLANSKA, P; ZACHOVA, D; VAGNEROVA, R; ANGELIS, KJ; FAJKUS, J, 2015:Telomere dynamics in the lower plant Physcomitrella patens. PLANT MOLECULAR BIOLOGY 87 (6), p. 591 - 601.
  • HEGER, Z; MICHALEK, P; GURAN, R; HAVELKOVA, B; KOMINKOVA, M; CERNEI, N; RICHTERA, L; BEKLOVA, M; ADAM, V; KIZEK, R, 2015:Exposure to 17 beta-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida. PLOS ONE 10 (12)
  • JANOUSKOVA, E; NECASOVA, I; PAVLOUSKOVA, J; ZIMMERMANN, M; HLUCHY, M; MARINI, V; NOVAKOVA, M; HOFR, C, 2015:Human Rap1 modulates TRF2 attraction to telomeric DNA. NUCLEIC ACIDS RESEARCH 43 (5), p. 2691 - 2700.
  • KOZAKOVA, L; VONDROVA, L; STEJSKAL, K; CHARALABOUS, P; KOLESAR, P; LEHMANN, AR; ULDRIJAN, S; SANDERSON, CM; ZDRAHAL, Z; PALECEK, JJ, 2015:The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex. CELL CYCLE 14 (6), p. 920 - 930.
  • MUCHOVA, V; AMIARD, S; MOZGOVA, I; DVORACKOVA, M; GALLEGO, ME; WHITE, C; FAJKUS, J, 2015:Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants. PLANT JOURNAL 81 (2), p. 198 - 209.
  • PALECEK, E; TKAC, J; BARTOSIK, M; BERTOK, T; OSTATNA, V; PALECEK, J, 2015:Electrochemistry of Nonconjugated Proteins and Glycoproteins. Toward Sensors for Biomedicine and Glycomics. CHEMICAL REVIEWS , p. 20452045 - 2108.
  • PESKA, V; FAJKUS, P; FOJTOVA, M; DVORACKOVA, M; HAPALA, J; DVORACEK, V; POLANSKA, P; LEITCH, AR; SYKOROVA, E; FAJKUS, J, 2015:Characterisation of an unusual telomere motif (TTTTTTAGGG)(n) in the plant Cestrum elegans (Solanaceae), a species with a large genome. PLANT JOURNAL 82 (4), p. 644 - 654.
  • TRAN, TD; CAO, HX; JOVTCHEV, G; NEUMANN, P; NOVAK, P; FOJTOVA, M; VU, GTH; MACAS, J; FAJKUS, J; SCHUBERT, I; FUCHS, J, 2015:Centromere and telomere sequence alterations reflect the rapid genome evolution within the carnivorous plant genus Genlisea. PLANT JOURNAL 84 (6), p. 1087 - 1099.
  • ZDARSKA, M; DOBISOVA, T; GELOVA, Z; PERNISOVA, M; DABRAVOLSKI, S; HEJATKO, J, 2015:Illuminating light, cytokinin, and ethylene signalling crosstalk in plant development. JOURNAL OF EXPERIMENTAL BOTANY 66 (16), p. 4913 - 4931.

2014

  • MAJEROVA, E; MANDAKOVA, T; VU, GTH; FAJKUS, J; LYSAK, MA; FOJTOVA, M, 2014:Chromatin features of plant telomeric sequences at terminal vs. internal positions. FRONTIERS IN PLANT SCIENCE 5 , p. 593 - .
  • OGROCKA, A; POLANSKA, P; MAJEROVA, E; JANEBA, Z; FAJKUS, J; FOJTOVA, M, 2014:Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants. NUCLEIC ACIDS RESEARCH 42 (5), p. 2919 - 2931.
  • SCHRUMPFOVA, PP; VYCHODILOVA, I; DVORACKOVA, M; MAJERSKA, J; DOKLADAL, L; SCHOROVA, S; FAJKUS, J, 2014:Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase. PLANT JOURNAL 77 (5), p. 770 - 781.

2013

  • CINCAROVA, L; ZDRAHAL, Z; FAJKUS, J, 2013:New perspectives of valproic acid in clinical practice. EXPERT OPINION ON INVESTIGATIONAL DRUGS 22 (12), p. 1535 - 1547.
  • PONTVIANNE, F; BLEVINS, T; CHANDRASEKHARA, C; MOZGOVA, I; HASSEL, C; PONTES, OMF; TUCKER, S; MOKROS, P; MUCHOVA, V; FAJKUS, J; PIKAARD, CS, 2013:Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states. GENES & DEVELOPMENT 27 (14), p. 1545 - 1550.

2012

  • CINCAROVA, L; LOCHMANOVA, G; NOVAKOVA, K; SULTESOVA, P; KONECNA, H; FAJKUSOVA, L; FAJKUS, J; ZDRAHAL, Z, 2012:A combined approach for the study of histone deacetylase inhibitors. MOLECULAR BIOSYSTEMS 8 (11), p. 2937 - 2945.
  • GUERINEAU, M; KRIZ, Z; KOZAKOVA, L; BEDNAROVA, K; JANOS, P; PALECEK, J, 2012:Analysis of the Nse3/MAGE-Binding Domain of the Nse4/EID Family Proteins. PLOS ONE 7 (4)
  • KUBICEK, K; CERNA, H; HOLUB, P; PASULKA, J; HROSSOVA, D; LOEHR, F; HOFR, C; VANACOVA, S; STEFL, R, 2012:Serine phosphorylation and proline isomerization in RNAP II CTD control recruitment of Nrd1. GENES & DEVELOPMENT 26 (17), p. 1891 - 1896.
  • LEE, LY; WU, FH; HSU, CT; SHEN, SC; YEH, HY; LIAO, DC; FANG, MJ; LIU, NT; YEN, YC; DOKLADAL, L; SYKOROVA, E; GELVIN, SB; LIN, CS, 2012:Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. PLANT CELL 24 (5), p. 1746 - 1759.
  • OGROCKA, A; SYKOROVA, E; FAJKUS, J; FOJTOVA, M, 2012:Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment. JOURNAL OF EXPERIMENTAL BOTANY 63 (11), p. 4233 - 4241.
  • POLANSKA, E; DOBSAKOVA, Z; DVORACKOVA, M; FAJKUS, J; STROS, M, 2012:HMGB1 gene knockout in mouse embryonic fibroblasts results in reduced telomerase activity and telomere dysfunction. CHROMOSOMA 121 (4), p. 419 - 431.
  • VISACKA, K; HOFR, C; WILLCOX, S; NECASOVA, I; PAVLOUSKOVA, J; SEPSIOVA, R; WIMMEROVA, M; SIMONICOVA, L; NOSEK, J; FAJKUS, J; GRIFFITH, JD; TOMASKA, L, 2012:Synergism of the Two Myb Domains of Tay1 Protein Results in High Affinity Binding to Telomeres. JOURNAL OF BIOLOGICAL CHEMISTRY 287 (38), p. 32206 - 32215.

2011

  • FOJTOVA, M; PESKA, V; DOBSAKOVA, Z; MOZGOVA, I; FAJKUS, J; SYKOROVA, E, 2011:Molecular analysis of T-DNA insertion mutants identified putative regulatory elements in the AtTERT gene. JOURNAL OF EXPERIMENTAL BOTANY 62 (15), p. 5531 - 5545.
  • HOBOR, F; PERGOLI, R; KUBICEK, K; HROSSOVA, D; BACIKOVA, V; ZIMMERMANN, M; PASULKA, J; HOFR, C; VANACOVA, S; STEFL, R, 2011:Recognition of Transcription Termination Signal by the Nuclear Polyadenylated RNA-binding (NAB) 3 Protein. JOURNAL OF BIOLOGICAL CHEMISTRY 286 (5), p. 3645 - 3657.
  • HUDSON, JJR; BEDNAROVA, K; KOZAKOVA, L; LIAO, CY; GUERINEAU, M; COLNAGHI, R; VIDOT, S; MAREK, J; BATHULA, SR; LEHMANN, AR; PALECEK, J, 2011:Interactions between the Nse3 and Nse4 Components of the SMC5-6 Complex Identify Evolutionarily Conserved Interactions between MAGE and EID Families. PLOS ONE 6 (2)
  • MAJEROVA, E; FOJTOVA, M; MOZGOVA, I; BITTOVA, M; FAJKUS, J, 2011:Hypomethylating drugs efficiently decrease cytosine methylation in telomeric DNA and activate telomerase without affecting telomere lengths in tobacco cells. PLANT MOLECULAR BIOLOGY 77 (42128), p. 371 - 380.

2010

  • RICHARD STEFL, FLORIAN C. OBERSTRASS, JENNIFER L. HOOD, MURIEL JOURDAN, MICHAL ZIMMERMANN, LENKA SKRISOVSKA, CHRISTOPHE MARIS, LI PENG, CTIRAD HOFR, RONALD B. EMESON, FRÉDÉRIC H.-T. ALLAINE, 2010:The solution structure of the ADAR2 dsRBM-RNA complex reveals a sequence-specific read out of the minor groove. CELL 143 (2), p. 225 - 237.

2009

  • HOFR, C., SULTESOVA, P., ZIMMERMANN, M., MOZGOVA, I., SCHRUMPFOVA, P. P., WIMMEROVA, M., FAJKUS, J.,, 2009:Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specificity and kinetics. BIOCHEMICAL JOURNAL 419 , p. 221 - 228.

GRANTY

  • Dynamika vzniku shelterinového komplexu (P205/12/0550), Czech Science Foundation - Standard Grants, 2012 - 2015
  • Telomeres and telomerase: transition from molecular to structural biology approach (IAA500040801), Academy of Sciences of the Czech Republic - Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Rep., 2008 - 2012
  • Epigenetické mechanismy regulace rostlinných telomer (GAP501/11/0569), Czech Science Foundation - Standard Grants, 2011 - 2015
  • Ztráta specifických repetic DNA v důsledku dysfunkce CAF1 u rostlin (GAP501/11/0289), Czech Science Foundation - Standard Grants, 2011 - 2015

CURRENT RESEARCH INFRASTRUCTURE

The current research infrastructure includes high-end microscopy, quantitative fluorescence spectroscopy, centrifugation and ultracentrifugation, preparative and analytical chromatography, electrophoresis, in situ and Southern hybridization, phosphofluoroimaging, real-time PCR and a documentation system for chemiluminiscence and fluorescence.

1. Molecular nature of evolutionary telomere changes

Supervisor: prof. RNDr. Jiří Fajkus, CSc.

Annotation:

The aim of the thesis will be to clarify molecular mechanisms of evolutionary telomere transitions that we have described earlier. Telomerase subunits potentially responsible for the changes will be characterised. Impact of the changes on function of telomerases and telomeres will be elucidated.

2. Role of the TRIM-MAGE complexes in cellular processes

Supervisor: doc. Mgr. Jan Paleček, Dr. rer. nat.
Consultant: Mgr. Stjepan Uldrijan, CSc.

Annotation:

More then 60 genes comprise the MAGE (Melanoma-associated antiGEn) superfamily in human genome. Most of them are expressed in specific cancer/testis manner. Recent findings suggest their role in tumorigenesis via their binding to TRIM proteins. These MAGE-TRIM complexes exhibit ubiquitin-ligase activities which facilitate degradation of key tumor suppressor proteins. In this project, student will use broad range of biochemical, molecular and cancer biology techniques to uncover molecular mechanisms of MAGE-TRIM-driven processes.

Literature:
KOZÁKOVÁ, L.;VONDROVÁ, L.;STEJSKAL, K.;CHARALABOUS, P.;KOLESÁR, P.;LEHMANN, A.R.;ULDRIJAN, S.;SANDERSON, Ch.M.;ZDRÁHAL, Z.;PALEČEK, J. The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex. Cell Cycle, Philadelphia: Taylor&Francis Inc, 2015, roč. 14, č. 6, s. 920-930. ISSN 1538-4101. doi:10.1080/15384101.2014.1000112. 

3.Telomeric chromatin in plants with non-canonical telomeres

Supervisor: Mgr. Miloslava Fojtová, CSc.
Consultant: Prof. RNDr. Jiří Fajkus, CSc.

Annotation:

Telomeres are nucleoprotein structures delimiting ends of linear eukaryotic chromosomes. They are crucial for the maintenance of genome integrity, distinguishing natural chromosome ends from DNA breaks and protecting genetic information settled inside chromosomes from the loss due to the incomplete replication of distal-most chromosome regions.

At the DNA level, telomeres consist of minisatellite repeats, TTTAGGG in most of plants. Nevertheless, plant representatives with non-canonical telomeric repeats were identified in our laboratory, including the carnivorous plant of the Genlisea family, G. hispidula (TTCAGG/TTTCAGG) and Allium species (CTCGGTTATGGG). Some specific features of the telomeric chromatin in model plants with canonical telomeres were shown, includingthe presence of methylated cytosines in the C-rich telomeric strand and bivalent nature of their chromatin with histones decorated with both heterochromatin- and euchromatin-specific modifications.

In this project, telomeric chromatin in plants with non-canonical telomeric sequences will be analysed with respect to their epigenetic modifications and capability to form higher- order structures. Student will be responsible for modification of standard molecular biology approaches for analysis of non-model plant species and for optimization of the method directly demonstrating presence of methylated cytosines in telomeric repeats. Characterisation of epigenetic properties of this essential functional genomic region will be supplemented by functional study in which distribution of epigenetic marks will be modulated either using epigenetically active chemicals, or genome editing-based methods.

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