Změnit instituci
Pokročilé nano a mikrotechnologie Pokročilé materiály Strukturní biologie Gen. a prot. rostlin. systémů Molekulární medicína Výzkum mozku a lidské mysli Molekulární vet. medicína

Výzkumná skupina Tomasze Nodzynskieho

Vedoucí výzkumné skupiny
Telefon: +420 54949 8420
E-mail:
Kancelář:

Research areas

Plants as sessile organisms must adapt to ever changing environmental conditions. The adaptations to environmental cues are essential for plant survival and consequently for crop productivity. The adaptation processes rely on sensing and transduction of environmental signals, integration of various forms of signals and determination of the final response. Plant hormones are implicated in various aspects of regulation of plant development, growth and responses to environmental cues. Our work is focused on systematic analysis of environmental and hormonal signaling in regulating plant growth and development, as well as adaptation to environmental cues.

We focus on how different environmental and endogenous signals are integrated into the subcellular dynamics and polar localisation of transporters for plant hormone auxin. We expect to get a deeper knowledge on plasticity of plant responses to environmental cues.

Main objectives

1. Plant hormonal signalling for regulation of cell polarity and subcellular dynamics:

Identification of key entry points by which auxin and other signalling pathways modulate subcellular dynamics and polar trafficking of PIN auxin transporters

2. Cell polarity and subcellular dynamics in plant cells:

We elucidate molecular and cellular mechanisms underlying cell polarity and trafficking processes by combination of genetics and high resolution microscopy approaches.

3. Perception of external signals and their integration into subcellular dynamics and cell polarity:

Using combination of reverse genetic, chemical genomics, life cell and high-resolution imaging approaches; we gain new insights into how gravity and light perception is integrated into regulation of subcellular dynamics.

4. Integration of hormonal signalling and subcellular dynamics for multicellular tissue development by mathematical modelling:

The acquired knowledge on hormonal networks; processes of subcellular dynamics and integration of different signals will be used to mathematically model these processes and to extrapolate them onto multicellular developmental situations.

seznam / vizitky

Jméno a pozice

E-mail

Telefon

Tomasz Nodzynski, B.A., M.Sc., Ph.D.
Vedoucí výzkumné skupiny
+420 54949 8420
Mgr. Eva Medvecká, PhD.
Odborná pracovnice ve výzkumu - postdoc
+420 54949 8422
Sibu Simon, Ph.D.
Odborný pracovník ve výzkumu - postdoc
+420 54949 3699
Marta Zwiewka, B.A., M.Sc., Ph.D.
Odborná pracovnice ve výzkumu - postdoc
+420 54949 8419
Mgr. Nikola Rýdza
Odborná pracovnice - PhD studentka
Lilla Koczka
Sravankumar Thula, M.Sc.
Odborný pracovník - PhD student
Mgr. Vendula Pukyšová
Odborná pracovnice - PhD studentka
Bc. Veronika Bilanovičová
Adria Sans Sánchez
Odborný pracovník - PhD student
Mgr. Stanislava Sladeček

VYBRANÉ PUBLIKACE

2016

  • BALLA, J; MEDVED'OVA, Z; KALOUSEK, P; MATIJESCUKOVA, N; FRIML, J; REINOHL, V; PROCHAZKA, S, 2016:Auxin flow-mediated competition between axillary buds to restore apical dominance. SCIENTIFIC REPORTS 6
  • NODZYNSKI, T; VANNESTE, S; ZWIEWKA, M; PERNISOVA, M; HEJATKO, J; FRIML, J, 2016:Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components. MOLECULAR PLANT 9 (11), p. 1504 - 1519.
  • PAVLISTOVA, V; DVORACKOVA, M; JEZ, M; MOZGOVA, I; MOKROS, P; FAJKUS, J, 2016:Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes. PLANT JOURNAL 88 (3), p. 411 - 424.
  • PERNISOVA, M; PRAT, T; GRONES, P; HARUSTIAKOVA, D; MATONOHOVA, M; SPICHAL, L; NODZYNSKI, T; FRIML, J; HEJATKO, J, 2016:Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis. NEW PHYTOLOGIST 212 (2), p. 497 - 509.
  • SIMON, S; SKUPA, P; VIAENE, T; ZWIEWKA, M; TEJOS, R; KLIMA, P; CARNA, M; ROLCIK, J; DE RYCKE, R; MORENO, I; DOBREV, PI; ORELLANA, A; ZAZIMALOVA, E; FRIML, J, 2016:PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. NEW PHYTOLOGIST 211 (1), p. 65 - 74.
  • ZHU, JS; BAILLY, A; ZWIEWKA, M; SOVERO, V; DI DONATO, M; GE, P; OEHRI, J; ARYAL, B; HAO, PC; LINNERT, M; BURGARDT, NI; LUCKE, C; WEIWAD, M; MICHEL, M; WEIERGRABER, OH; POLLMANN, S; AZZARELLO, E; MANCUSO, S; FERRO, N; FUKAO, Y; HOFFMANN, C; WEDLICH-SOLDNER, R; FRIML, J; THOMAS, C; GEISLER, M, 2016:TWISTED DWARF1 Mediates the Action of Auxin Transport Inhibitors on Actin Cytoskeleton Dynamics. PLANT CELL 28 (4), p. 930 - 948.

2015

  • GRONES, P; FRIML, J, 2015:ABP1: Finally Docking. MOLECULAR PLANT 8 (3), p. 356 - 358.
  • GRONES, P; CHEN, X; SIMON, S; KAUFMANN, WA; DE RYCKE, R; NODZYNSKI, T; ZAZIMALOVA, E; FRIML, J, 2015:Auxin-binding pocket of ABP1 is crucial for its gain-of-function cellular and developmental roles. JOURNAL OF EXPERIMENTAL BOTANY 66 (16), p. 5055 - 5065.
  • ROBERT, HS; GRUNEWALD, W; SAUER, M; CANNOOT, B; SORIANO, M; SWARUP, R; WEIJERS, D; BENNETT, M; BOUTILIER, K; FRIML, J, 2015:Plant embryogenesis requires AUX/LAX-mediated auxin influx. DEVELOPMENT 142 (4), p. 702 - 711.
  • ZWIEWKA, M; NODZYNSKI, T; ROBERT, S; VANNESTE, S; FRIML, J, 2015:Osmotic Stress Modulates the Balance between Exocytosis and Clathrin-Mediated Endocytosis in Arabidopsis thaliana. MOLECULAR PLANT 8 (8), p. 1175 - 1187.

2014

  • BAILLY, A; WANG, BJ; ZWIEWKA, M; POLLMANN, S; SCHENCK, D; LUTHEN, H; SCHULZ, A; FRIML, J; GEISLER, M, 2014:Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. PLANT JOURNAL 77 (1), p. 108 - 118.
  • CHEN, YN; AUNG, K; ROLCIK, J; WALICKI, K; FRIML, J; BRANDIZZI, F, 2014:Inter-regulation of the unfolded protein response and auxin signaling. PLANT JOURNAL 77 (1), p. 97 - 107.
  • NARAMOTO, S; NODZYNSKI, T; DAINOBU, T; TAKATSUKA, H; OKADA, T; FRIML, J; FUKUDA, H, 2014:VAN4 Encodes a Putative TRS120 That is Required for Normal Cell Growth and Vein Development in Arabidopsis. PLANT AND CELL PHYSIOLOGY 55 (4), p. 750 - 763.
  • SAUER, M; FRIML, J, 2014:Plant Biology: Gatekeepers of the Road to Protein Perdition. CURRENT BIOLOGY 24 (1), p. R27 - R29.
  • XU, TD; DAI, N; CHEN, JS; NAGAWA, S; CAO, M; LI, HJ; ZHOU, ZM; CHEN, X; DE RYCKE, R; RAKUSOVA, H; WANG, WY; JONES, AM; FRIML, J; PATTERSON, SE; BLEECKER, AB; YANG, ZB, 2014:Cell Surface ABP1-TMK Auxin-Sensing Complex Activates ROP GTPase Signaling. SCIENCE 343 (6174), p. 1025 - 1028.

2013

  • COVANOVA, M; SAUER, M; RYCHTAR, J; FRIML, J; PETRASEK, J; ZAZIMALOVA, E, 2013:Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells. PLOS ONE 8 (7)
  • PONTVIANNE, F; BLEVINS, T; CHANDRASEKHARA, C; MOZGOVA, I; HASSEL, C; PONTES, OMF; TUCKER, S; MOKROS, P; MUCHOVA, V; FAJKUS, J; PIKAARD, CS, 2013:Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states. GENES & DEVELOPMENT 27 (14), p. 1545 - 1550.
  • ROBERT, HS; GRONES, P; STEPANOVA, AN; ROBLES, LM; LOKERSE, AS; ALONSO, JM; WEIJERS, D; FRIML, J, 2013:Local Auxin Sources Orient the Apical-Basal Axis in Arabidopsis Embryos. CURRENT BIOLOGY 23 (24), p. 2506 - 2512.
  • SIMON, S; KUBES, M; BASTER, P; ROBERT, S; DOBREV, PI; FRIML, J; PETRASEK, J; ZAZIMALOVA, E, 2013:Defining the selectivity of processes along the auxin response chain: a study using auxin analogues. NEW PHYTOLOGIST 200 (4), p. 1034 - 1048.
  • WABNIK, K; ROBERT, HS; SMITH, RS; FRIML, J, 2013:Modeling Framework for the Establishment of the Apical-Basal Embryonic Axis in Plants. CURRENT BIOLOGY 23 (24), p. 2513 - 2518.

2011

  • WABNIK, K; KLEINE-VEHN, J; GOVAERTS, W; FRIML, J, 2011:Prototype cell-to-cell auxin transport mechanism by intracellular auxin compartmentalization. TRENDS IN PLANT SCIENCE 16 (9), p. 468 - 475.

GRANTY

  • Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách (GA13-40637S), Czech Science Foundation - Standard Grants, 2013 - 2017
  • Multi-disciplinary approach in strigolactone research to battle crop infestation by parasitic Striga sp (5SA14534), South Moravian Region - SoMoPro, 2016 - 2019

1. Evolution of auxin transport and cell polarity mechanisms in plant lineage

Supervisor: prof. Jiří Friml, Dr.

Annotation:

Plant development is characterized by a remarkable adaptability to different environmental conditions. Plants have the flexibility to re-organize post-embryonic development through regulation of asymmetric distributions (gradients) of the plant growth regulator auxin. Auxin is unique among plant signaling molecules, because it is polarly transported through plant tissues and its transport largely depends on subcellular localization of auxin efflux carriers – PIN proteins. Our lab aims to understand mechanisms of PIN polarization and auxin-mediated plant morphogenesis using experimental and theoretical approaches. This PhD project focuses on how the unique, plant-specific mechanisms of auxin transport and polar trafficking appeared and evolved in the plant lineage. The project will involve a combination of molecular genetic, cell biological and biochemistry techniques in early diverging plants such as moss Physcomitrella patents and filamentous algae as well as in the model plant Arabidopsis thaliana. We expect: Highly motivated candidates with ambition of producing relevant top results. We offer: Experimental work on own project with the possibility of publication in top international journals, world-class lab equipment, international cooperative team, close cooperation with abroad laboratories, competitive salary corresponding to the work efficiency. Adamowski M, Friml J. (2015): PIN-Dependent Auxin Transport: Action, Regulation, and Evolution. Plant Cell. 27(1):20-32. Viaene T, Landberg K, Thelander M, Medvecka E, Pederson E, Feraru E, Cooper ED, Karimi M, Delwiche CF, Ljung K, Geisler M, Sundberg E, Friml J. (2014): Directional Auxin Transport Mechanisms in Early Diverging Land Plants. Curr Biol. 23: 2786-2791 Mravec J, Skůpa P, Bailly A, Křeček P, Hoyerová K, Bielach A, Petrášek J, Zhang J, Gaykova V, Stierhof Y-D, Schwarzerová K, Rolčík J, Dobrev P, Seifertová D, Luschnig C, Benková E, Zažímalová E, Geisler M, Friml J. (2009): ER localized PIN5 auxin transporter mediates subcellular homeostasis of phytohormone auxin. Nature. 459(7250):1136-40

2. Biochemical and structural insights into PIN-mediated auxin transport

Supervisor: Tomasz Nodzynski, B.A., M.Sc., Ph.D.
Consultants: Prof. Jiří Friml

Annotation:

The signalling molecule auxin provides instructive cues guiding the entire plant ontogenesis. Its orchestrating role largely depends on the directional transport between cells facilitated by a plant-specific group of auxin transporters named PINs. While the physiological and developmental roles of PINs along with their intracellular dynamics have been extensively studied, the biochemical and structural underpinnings of PIN activity are only scarcely characterized. Proposed research aims to fill this gap providing a detailed biochemical characterisation of auxin transport, including PIN structure-function aspects responsible for transport and auxin binding specificity. Obtained results will be described using mathematical models allowing for more accurate results interpretation and detailed linking of PIN biochemistry and function. Our findings have also a clear application potential to engineer auxin-based aspects of physiology and architecture in the important plant species as well as help in the development of new auxin derivatives applicable as modern tools in crop cultivation.

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