Změnit instituci
Pokročilé nano a mikrotechnologie Pokročilé materiály Strukturní biologie Gen. a prot. rostlin. systémů Molekulární medicína Výzkum mozku a lidské mysli Molekulární vet. medicína

Výzkumná skupina Zbyňka Zdráhala

Vedoucí výzkumné skupiny
Researcher ID
Telefon: +420 54949 8258
Mobil: 777926602
E-mail: ,
Kancelář:
Zástupce vedoucího výzkumné skupiny
Researcher ID
Telefon: +420 54949 7304, +420 54949 8427
E-mail: ,
Kancelář:

Research areas

  • Separation of complex protein mixtures
  • Characterization of proteins and their modifications by mass spectrometry
  • Mass spectrometric techniques for the quantification of protein mixtures
  • MS-based proteomics in general

Main objectives

  • The development of novel techniques for the separation and characterization of proteins using mass spectrometry.

Content of research

Our research interests focus on the field of MS-based proteomics including protein isolation from various matrices, fractionation and separation of complex protein mixtures by chromatographic and electrophoretic techniques, namely on applications of mass spectrometric techniques for qualitative and quantitative characterization of proteins and modifications of them.

At present, we participate in several research projects with diverse topics in areas of molecular biology, biomedicine and agriculture. Our roles within these projects include activities in the development and aplication of MALDI-MS profiling for taxonomic studies of selected bacterial genera and funghi, characterization of differentially expressed proteins (e.g. plant proteins after hormonal treatment), characterization of protein complexes, characterization of histone acetylations and assessments of the influences of selected histone deacetylase inhibitors on histone acetylation states, characterization of phosphorylation states of selected proteins, characterization of groups of patatin proteins in different potato cultivars, and the search for potentional disease biomarkers in blood plasma.

 

seznam / vizitky

Jméno a pozice

E-mail

Telefon

doc. RNDr. Zbyněk Zdráhal, Dr.
Vedoucí výzkumné skupiny
+420 54949 8258
RNDr. Erika Lattová, PhD.
Vedoucí výzkumná pracovnice
+420 54949 8425
Mgr. Ondrej Šedo, Ph.D.
Vedoucí výzkumný pracovník
+420 54949 7304, +420 54949 8427
Mgr. Gabriela Lochmanová, Ph.D.
Odborná pracovnice ve výzkumu
+420 54949 8430, +420 54949 4721
Mgr. David Potěšil, Ph.D.
Odborný pracovník ve výzkumu
+420 54949 8426, +420 54949 8428
Danuše Fridrichová
Technička
+420 54949 8425
Mgr. Ondřej Bočánek
Odborný pracovník - PhD student
+420 54949 8442
Ing. Martina Grochalová
Odborná pracovnice - PhD studentka
+420 54949 4721
Mgr. Kateřina Hanáková
Odborná pracovnice - PhD studentka
+420 54949 8442
RNDr. Ivana Ihnatová
Odborná pracovnice - PhD studentka
+420 54949 8442
Mgr. Petra Ježková
PhD studentka
Mgr. Hana Kuchaříková
PhD studentka
Ing. Dominika Ledvinová
Odborná pracovnice - PhD studentka
+420 54949 8442
Mgr. Kamil Mikulášek
Odborný pracovník - PhD student
+420 54949 8442
Mgr. Michal Obořil
Odborný pracovník - PhD student

VYBRANÉ PUBLIKACE

2017

  • BOČÁNEK, O; ŠEDO, O; PEKÁR, S; ZDRÁHAL, Z, 2017:Evaluation of sample preparation protocols for spider venom profiling by MALDI-TOF MS. TOXICON 133 , p. 18 - 25.
  • GROCHALOVA, M; KONECNA, H; STEJSKAL, K; POTESIL, D; FRIDRICHOVA, D; SRBOVA, E; ORNEROVA, K; ZDRAHAL, Z, 2017:Deep coverage of the beer proteome. JOURNAL OF PROTEOMICS 162 , p. 119 - 124.
  • JANSEN, S; MELKOVA, K; TROSANOVA, Z; HANAKOVA, K; ZACHRDLA, M; NOVACEK, J; ZUPA, E; ZDRAHAL, Z; HRITZ, J; ZIDEK, L, 2017:Quantitative mapping of microtubule-associated protein 2c (MAP2c) phosphorylation and regulatory protein 14-3-3 zeta-binding sites reveals key differences between MAP2c and its homolog Tau. JOURNAL OF BIOLOGICAL CHEMISTRY 292 (16), p. 6715 - 6727.
  • MAJERSKA, J; SCHRUMPFOVA, PP; DOKLADAL, L; SCHOROVA, S; STEJSKAL, K; OBORIL, M; HONYS, D; KOZAKOVA, L; POLANSKA, PS; SYKOROVA, E, 2017:Tandem affinity purification of AtTERT reveals putative interaction partners of plant telomerase in vivo. PROTOPLASMA 254 (4), p. 1547 - 1562.

2016

  • CERVENKA, I; VALNOHOVA, J; BERNATIK, O; HARNOS, J; RADSETOULAL, M; SEDOVA, K; HANAKOVA, K; POTESIL, D; SEDLACKOVA, M; SALASOVA, A; STEINHART, Z; ANGERS, S; SCHULTE, G; HAMPL, A; ZDRAHAL, Z; BRYJA, V, 2016:Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113 (33), p. 9304 - 9309.
  • HAFIDH, S; POTESIL, D; FILA, J; CAPKOVA, V; ZDRAHAL, Z; HONYS, D, 2016:Quantitative proteomics of the tobacco pollen tube secretome identifies novel pollen tube guidance proteins important for fertilization. GENOME BIOLOGY 17
  • LATTOVA, E; BRYANT, J; SKRICKOVA, J; ZDRAHAL, Z; POPOVIC, M, 2016:Efficient Procedure for N-Glycan Analyses and Detection of Endo H-Like Activity in Human Tumor Specimens. JOURNAL OF PROTEOME RESEARCH 15 (8), p. 2777 - 2786.
  • MIKULASEK, K; JARON, KS; KULHANEK, P; BITTOVA, M; HAVLIS, J, 2016:Sequence-dependent separation of trinucleotides by ion-interaction reversed-phase liquid chromatography A structure-retention study assisted by soft-modelling and molecular dynamics. JOURNAL OF CHROMATOGRAPHY A 1469 , p. 88 - 95.
  • SEDO, O; KORAN, M; JAKESOVA, M; MIKULIKOVA, R; BOHAC, M; ZDRAHAL, Z, 2016:Rapid assignment of malting barley varieties by matrix-assisted laser desorption-ionisation - Time-of-flight mass spectrometry. FOOD CHEMISTRY 206 , p. 124 - 130.
  • SPONER, JE; SPONER, J; NOVAKOVA, O; BRABEC, V; SEDO, O; ZDRAHAL, Z; COSTANZO, G; PINO, S; SALADINO, R; DI MAURO, E, 2016:Emergence of the First Catalytic Oligonucleotides in a Formamide-Based Origin Scenario. CHEMISTRY-A EUROPEAN JOURNAL 22 (11), p. 3572 - 3586.

2015

  • DADAKOVA, K; HAVELKOVA, M; KURKOVA, B; TLOLKOVA, I; KASPAROVSKY, T; ZDRAHAL, Z; LOCHMAN, J, 2015:Proteome and transcript analysis of Vitis vinifera cell cultures subjected to Botrytis cinerea infection. JOURNAL OF PROTEOMICS 119 , p. 143 - 153.
  • HROSSOVA, D; SIKORSKY, T; POTESIL, D; BARTOSOVIC, M; PASULKA, J; ZDRAHAL, Z; STEFL, R; VANACOVA, S, 2015:RBM7 subunit of the NEXT complex binds U-rich sequences and targets 3'-end extended forms of snRNAs. NUCLEIC ACIDS RESEARCH 43 (8), p. 4236 - 4248.
  • KOZAKOVA, L; VONDROVA, L; STEJSKAL, K; CHARALABOUS, P; KOLESAR, P; LEHMANN, AR; ULDRIJAN, S; SANDERSON, CM; ZDRAHAL, Z; PALECEK, JJ, 2015:The melanoma-associated antigen 1 (MAGEA1) protein stimulates the E3 ubiquitin-ligase activity of TRIM31 within a TRIM31-MAGEA1-NSE4 complex. CELL CYCLE 14 (6), p. 920 - 930.
  • KREJCI, J; STIXOVA, L; PAGACOVA, E; LEGARTOVA, S; KOZUBEK, S; LOCHMANOVA, G; ZDRAHAL, Z; SEHNALOVA, P; DABRAVOLSKI, S; HEJATKO, J; BARTOVA, E, 2015:Post-Translational Modifications of Histones in Human Sperm. JOURNAL OF CELLULAR BIOCHEMISTRY 116 (10), p. 2195 - 2209.
  • LATTOVA, E; BRABCOVA, A; BARTOVA, V; POTESIL, D; BARTA, J; ZDRAHAL, Z, 2015:N-Glycome Profiling of Patatins from Different Potato Species of Solanum Genus. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 63 (12), p. 3243 - 3250.
  • NEMEC, A; KRIZOVA, L; MAIXNEROVA, M; SEDO, O; BRISSE, S; HIGGINS, PG, 2015:Acinetobacter seifertii sp nov., a member of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex isolated from human clinical specimens. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65 , p. 934 - 942.

2014

  • BALAZOVA, T; MAKOVCOVA, J; SEDO, O; SLANY, M; FALDYNA, M; ZDRAHAL, Z, 2014:The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling. FEMS MICROBIOLOGY LETTERS 353 (1), p. 77 - 84.
  • BALAZOVA, T; SEDO, O; STEFANIC, P; MANDIC-MULEC, I; VOS, M; ZDRAHAL, Z, 2014:Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion. RAPID COMMUNICATIONS IN MASS SPECTROMETRY 28 (17), p. 1855 - 1861.
  • BERNATIK, O; SEDOVA, K; SCHILLE, C; GANJI, RS; CERVENKA, I; TRANTIREK, L; SCHAMBONY, A; ZDRAHAL, Z; BRYJA, V, 2014:Functional Analysis of Dishevelled-3 Phosphorylation Identifies Distinct Mechanisms Driven by Casein Kinase 1 epsilon and Frizzled5. JOURNAL OF BIOLOGICAL CHEMISTRY 289 (34), p. 23520 - 23533.
  • DE GROOT, REA; GANJI, RS; BERNATIK, O; LLOYD-LEWIS, B; SEIPEL, K; SEDOVA, K; ZDRAHAL, Z; DHOPLE, VM; DALE, TC; KORSWAGEN, HC; BRYJA, V, 2014:Huwe1-Mediated Ubiquitylation of Dishevelled Defines a Negative Feedback Loop in the Wnt Signaling Pathway. SCIENCE SIGNALING 7 (317)
  • HAFIDH, S; POTESIL, D; FILA, J; FECIKOVA, J; CAPKOVA, V; ZDRAHAL, Z; HONYS, D, 2014:In search of ligands and receptors of the pollen tube: the missing link in pollen tube perception. BIOCHEMICAL SOCIETY TRANSACTIONS 42 , p. 388 - 394.

2013

  • CINCAROVA, L; ZDRAHAL, Z; FAJKUS, J, 2013:New perspectives of valproic acid in clinical practice. EXPERT OPINION ON INVESTIGATIONAL DRUGS 22 (12), p. 1535 - 1547.
  • KONECNA, H; FRIDRICHOVA, D; LOCHMANOVA, G; BARTA, J; ZDRAHAL, Z, 2013:Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels. ELECTROPHORESIS 34 (13), p. 1969 - 1971.
  • SEDO, O; NEMEC, A; KRIZOVA, L; KACALOVA, M; ZDRAHAL, Z, 2013:Improvement of MALDI-TOF MS profiling for the differentiation of species within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. SYSTEMATIC AND APPLIED MICROBIOLOGY 36 (8), p. 572 - 578.
  • STEJSKAL, K; POTESIL, D; ZDRAHAL, Z, 2013:Suppression of Peptide Sample Losses in Autosampler Vials. JOURNAL OF PROTEOME RESEARCH 12 (6), p. 3057 - 3062.
  • ZD'ARSKA, M; ZATLOUKALOVA, P; BENITEZ, M; SEDO, O; POTESIL, D; NOVAK, O; SVACINOVA, J; PESEK, B; MALBECK, J; VASICKOVA, J; ZDRAHAL, Z; HEJATKO, J, 2013:Proteome Analysis in Arabidopsis Reveals Shoot- and Root-Specific Targets of Cytokinin Action and Differential Regulation of Hormonal Homeostasis. PLANT PHYSIOLOGY 161 (2), p. 918 - 930.

2012

  • BARTA, J; BARTOVA, V; ZDRAHAL, Z; SEDO, O, 2012:Cultivar Variability of Patatin Biochemical Characteristics: Table versus Processing Potatoes (Solanum tuberosum L.). JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60 (17), p. 4369 - 4378.
  • CINCAROVA, L; LOCHMANOVA, G; NOVAKOVA, K; SULTESOVA, P; KONECNA, H; FAJKUSOVA, L; FAJKUS, J; ZDRAHAL, Z, 2012:A combined approach for the study of histone deacetylase inhibitors. MOLECULAR BIOSYSTEMS 8 (11), p. 2937 - 2945.
  • DOKLADAL, L; OBORIL, M; STEJSKAL, K; ZDRAHAL, Z; PTACKOVA, N; CHALOUPKOVA, R; DAMBORSKY, J; KASPAROVSKY, T; JEANDROZ, S; ZD'ARSKA, M; LOCHMAN, J, 2012:Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. JOURNAL OF EXPERIMENTAL BOTANY 63 (5), p. 2203 - 2215.
  • KONECNA, H; MULLER, L; DOSOUDILOVA, H; POTESIL, D; BURSIKOVA, J; SEDO, O; MAROVA, I; ZDRAHAL, Z, 2012:Exploration of Beer Proteome Using OFFGEL Prefractionation in Combination with Two-Dimensional Gel Electrophoresis with Narrow pH Range Gradients. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 60 (10), p. 2418 - 2426.
  • PEKAR, S; SMERDA, J; HRUSKOVA, M; SEDO, O; MUSTER, C; CARDOSO, P; ZDRAHAL, Z; KORENKO, S; BURES, P; LIZNAROVA, E; SENTENSKA, L, 2012:Prey-race drives differentiation of biotypes in ant-eating spiders. JOURNAL OF ANIMAL ECOLOGY 81 (4), p. 838 - 848.
  • SEDO, O; MAROVA, I; ZDRAHAL, Z, 2012:Beer fingerprinting by Matrix-Assisted Laser Desorption-Ionisation-Time of Flight Mass Spectrometry. FOOD CHEMISTRY 135 (2), p. 473 - 478.

2011

  • NOVAKOVA, K; SEDO, O; ZDRAHAL, Z, 2011:Mass Spectrometry Characterization of Plant Phosphoproteins. CURRENT PROTEIN & PEPTIDE SCIENCE 12 (2), p. 112 - 125.
  • SEDO, O; SEDLACEK, I; ZDRAHAL, Z, 2011:SAMPLE PREPARATION METHODS FOR MALDI-MS PROFILING OF BACTERIA. MASS SPECTROMETRY REVIEWS 30 (3), p. 417 - 434.
  • SEDO, O; VORAC, A; ZDRAHAL, Z, 2011:Optimization of mass spectral features in MALDI-TOF MS profiling of Acinetobacter species. SYSTEMATIC AND APPLIED MICROBIOLOGY 34 (1), p. 30 - 34.

GRANTY

  • RNA vazebné proteiny účastnící se translační represe skladovaných transkriptů během vývoje samčího gametofytu (GA15-16050S), Czech Science Foundation - Standard Grants, 2015 - 2017
  • Signalizace mezi pylem a pestíkem předcházející oplození; úloha sekretovaných signálů působících na kratší a delší vzdálenost (GA15-22720S), Czech Science Foundation - Standard Grants, 2015 - 2017
  • Centrum nových přístupů k bioanalýze a molekulární diagnostice (P206/12/G151), Czech Science Foundation - Projects for promotion of excellence in basic research, 2012 - 2018
  • Metody tlumení produkčních chorob skotu - BVD-MD a paratuberkulóza (QI101A094), Ministry of Agriculture - The Research Program in the Agricultural Sector, 2010 - 2014

CURRENT RESEARCH INFRASTRUCTURE

The current infrastructure includes instrumentation for protein isolation, protein separation and mass spectrometry.

Key instruments: OrbitrapElite hybrid mass spectrometer

1. Proteomics of the lysine acetylome in cancer cells

Supervisor: doc. RNDr. Zbyněk Zdráhal, Dr.
Consultants: Mgr. Gabriela Lochmanová, Ph.D.

Annotation:

Lysine acetylation represents a dynamic post-translational modification (PTM) which in context of other PTMs increases the functional diversity of proteome. While acetylation has been extensively studied in core histones, cytoplasmic proteins have been reported to be acetylated, too; indicating that acetylation plays key role in a variety of signalling and regulatory pathways. Targeting protein acetylation represents a potentially promising strategy for disease treatment, such as cancer. The aim of the doctoral study is to characterize global protein acetylation in cancer cells by mass spectrometry-based methods. As analysis of acetylome is challenging due to overall low abundance of acetylated proteins, the suggested PhD topic will address development of the methodology consisting of acetylated proteins / peptides enrichment, separation of enriched peptides, and introducing an approach for determining levels of individual acetylated peptides based either on isotopic labelling or label-free quantification. Experimental part of study will be performed in CEITEC proteomics facility. Candidates who are interested in the topic are requested to contact supervisor of the project in advance –Zbyněk Zdráhal (zdrahal@sci.muni.cz).

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