Změnit instituci
Pokročilé nano a mikrotechnologie Pokročilé materiály Strukturní biologie Gen. a prot. rostlin. systémů Molekulární medicína Výzkum mozku a lidské mysli Molekulární vet. medicína

Zoonózy

Vedoucí výzkumné skupiny

Research areas

  • Analysis of the aspects associated with diversity and host specificity of selected model groups of parasitic pathogens, their zoonotic potential and the risk of infectious diseases for human and domestic and wild animals emerging
  • Development of specific and sensitive molecular tools for the diagnosis of toxoplasmosis
  • Proteomic analysis of the excretory-secretory proteins of the Trichinella species and identification of key parasite proteins that are involved in the host-parasite interaction

Main objectives

  • To obtain novel information on the causes, mechanisms and spreading of infectious diseases in domestic animals.
  • Identification of mechanisms and prevention of the circulation of zoonotic pathogens and commensal antimicrobial-resistant bacteria in the food chain.

 

Content of research

Analysis of the causes, mechanisms and spread of infectious diseases in domestic animals

Emerging diseases on the human-domestic animals - wildlife interface

The aim is to study principles underlying epidemiology of selected parasitic pathogens transmissible between man and animals, including improving the methods of the detection, understanding the links between molecular diversity and host specificity of selected model organisms, with emphasis on zoonotic potential.

Model situation 1: Piroplasma (Babesia, Theileria spp). Elucidation of host specificity and description of the barriers that prohibit the transmission of individual species / genotypes between various hosts.

Model situation 2: Protistan and helminth parasites of primates and man. The intimate evolutionary link between African great apes, such as, chimpanzees and gorillas, and humans creates a unique model situation, in which we can trace the history and origin of several human infections, i.e. from the perspective of interspecific boundaries and their crossing by pathogens. This part of the project is aimed at comparing communities of protozoan and helminth parasites in population of wild gorillas and chimpanzees on the level of individuals, i.e. infracommunities, as well as host populations and to apply the molecular tools to assess the genetic diversity of studied pathogens on infra-specific level and to analyse the consequences of observed transmission patterns for emergence of diseases, but also for conservation purposes.

Model situation 3: Parasitic and viral infection in isolated populations of African domestic dogs. The study focuses on the population of domesticated animals, namely dogs, lives without prophylactic or therapeutic interventions, being exposed to direct selection pressure of complex of pathogens. The overall aim of this part is to characterise infectious diseases in native dogs living so far without any veterinary care with Samburu/Turkana pastoralists in Turkana region N. Kenya.

Analysis and the prevention of the circulation of zoonotic pathogens in the food chain

Emerging food-borne parasites

This work package focuses mainly on nucleic acid-based and proteomic approaches for the diagnosis of the selected food-borne parasites and analysis of genetic variation among them. Among the major food-borne parasites are T. gondii and Trichinella spp. Based on two model situations, the work package aims to contribute to understanding the principles underlying the epidemiology of selected food-borne parasites transmissible between man and animals, including improving the methods of the detection.

Model situation 1: Toxoplasma gondii. Toxoplasmosis. Sera from food domestic and game animals will be tested for antibodies to T. gondii. T. gondii isolates from seropositive animals will be then genotyped. Genotyping data will be analysed to demonstrate the genetic diversity and geographical distribution of T. gondii in the Czech Republic.

Model situation 2: Trichinella. A global proteomics approach will be used to analyse the E/S proteins from T. spiralis muscle larvae. Specific Trichinella EST databases will be used to analyse the data.

Host genetics and comparative immugenomics

Genetic diversity and host-pathogen interactions in specific populations of domestic dogs

Several hundred samples from Kenyan village dogs will be available for genetic diversity and association analysis of various populations and sub-populations of these dogs. For these purposes, selected infectious / parasitic diseases will be diagnosed by molecular and serological techniques: rabies, distemper and several parasite species and phenotypic classification will be made.

seznam / vizitky

Jméno a pozice

E-mail

Telefon

prof. MVDr. Břetislav Koudela, CSc.
Vedoucí výzkumné skupiny
Prof. MVDr. David Modrý, Ph.D.
Senior researcher
MVDr. Lada Hofmannová, Ph.D.
Junior researcher
MVDr. Jana Petrášová, Ph.D.
Junior researcher
Mgr. Martina Gallusová
MVDr. Barbora Mitková
Kateřina Špůrková

VYBRANÉ PUBLIKACE

2016

  • GOMEZ, A; PETRZELKOVA, KJ; BURNS, MB; YEOMAN, CJ; AMATO, KR; VLCKOVA, K; MODRY, D; TODD, A; ROBINSON, CAJ; REMIS, MJ; TORRALBA, MG; MORTON, E; UMANA, JD; CARBONERO, F; GASKINS, HR; NELSON, KE; WILSON, BA; STUMPF, RM; WHITE, BA; LEIGH, SR; BLEKHMAN, R, 2016:Gut Microbiome of Coexisting BaAka Pygmies and Bantu Reflects Gradients of Traditional Subsistence Patterns. CELL REPORTS 14 (9), p. 2142 - 2153.
  • GOMEZ, A; ROTHMAN, JM; PETRZELKOVA, K; YEOMAN, CJ; VLCKOVA, K; UMANA, JD; CARR, M; MODRY, D; TODD, A; TORRALBA, M; NELSON, KE; STUMPF, RM; WILSON, BA; BLEKHMAN, R; WHITE, BA; LEIGH, SR, 2016:Temporal variation selects for diet-microbe co-metabolic traits in the gut of Gorilla spp (vol 10, pg 514, 2016). ISME JOURNAL 10 (2), p. 532 - 532.
  • GOMEZ, A; ROTHMAN, JM; PETRZELKOVA, K; YEOMAN, CJ; VLCKOVA, K; UMANA, JD; CARR, M; MODRY, D; TODD, A; TORRALBA, M; NELSON, KE; STUMPF, RM; WILSON, BA; BLEKHMAN, R; WHITE, BA; LEIGH, SR, 2016:Temporal variation selects for diet-microbe co-metabolic traits in the gut of Gorilla spp. ISME JOURNAL 10 (2), p. 514 - 526.
  • IONICA, AM; MATEI, IA; D'AMICO, G; DASKALAKI, AA; JURANKOVA, J; IONESCU, DT; MIHALCA, AD; MODRY, D; GHERMAN, CM, 2016:Role of golden jackals (Canis aureus) as natural reservoirs of Dirofilaria spp. in Romania. PARASITES & VECTORS 9
  • KALOUSOVA, B; HASEGAWA, H; PETRZELKOVA, KJ; SAKAMAKI, T; KOORIYMA, T; MODRY, D, 2016:Adult hookworms (Necator spp.) collected from researchers working with wild western lowland gorillas. PARASITES & VECTORS 9
  • KVAC, M; HAVRDOVA, N; HLASKOVA, L; DANKOVA, T; KANDERA, J; JEZKOVA, J; VITOVEC, J; SAK, B; ORTEGA, Y; XIAO, LH; MODRY, D; CHELLADURAI, JRJJ; PRANTLOVA, V; MCEVOY, J, 2016:Cryptosporidium proliferans n. sp (Apicomplexa: Cryptosporidiidae): Molecular and Biological Evidence of Cryptic Species within Gastric Cryptosporidium of Mammals. PLOS ONE 11 (1)
  • MAPUA, MI; PETRZELKOVA, KJ; BURGUNDER, J; DADAKOVA, E; BROZOVA, K; HRAZDILOVA, K; STEWART, FA; PIEL, AK; VALLO, P; FUEHRER, HP; HASHIMOTO, C; MODRY, D; QABLAN, MA, 2016:A comparative molecular survey of malaria prevalence among Eastern chimpanzee populations in Issa Valley (Tanzania) and Kalinzu (Uganda). MALARIA JOURNAL 15
  • MATEI, IA; D'AMICO, G; YAO, PK; IONICA, AM; KANYARI, PWN; DASKALAKI, AA; DUMITRACHE, MO; SANDOR, AD; GHERMAN, CM; QABLAN, M; MODRY, D; MIHALCA, AD, 2016:Molecular detection of Anaplasma platys infection in free-roaming dogs and ticks from Kenya and Ivory Coast. PARASITES & VECTORS 9
  • MIHALCA, AD; IONICA, AM; D'AMICO, G; DASKALAKI, AA; DEAK, G; MATEI, IA; SIMONCA, V; IORDACHE, D; MODRY, D; GHERMAN, CM, 2016:Thelazia callipaeda in wild carnivores from Romania: new host and geographical records. PARASITES & VECTORS 9
  • NECESANKOVA, M; VYCHODILOVA, L; ALBRECHTOVA, K; KENNEDY, LJ; HLAVAC, J; SEDLAK, K; MODRY, D; JANOVA, E; VYSKOCIL, M; HORIN, P, 2016:MYD88 and functionally related genes are associated with multiple infections in a model population of Kenyan village dogs. MOLECULAR BIOLOGY REPORTS 43 (12), p. 1451 - 1463.
  • VLCKOVA, K; GOMEZ, A; PETRZELKOVA, KJ; WHITTIER, CA; TODD, AF; YEOMAN, CJ; NELSON, KE; WILSON, BA; STUMPF, RM; MODRY, D; WHITE, BA; LEIGH, SR, 2016:Effect of Antibiotic Treatment on the Gastrointestinal Microbiome of Free-Ranging Western Lowland Gorillas (Gorilla g. gorilla). MICROBIAL ECOLOGY 72 (4), p. 943 - 954.

2015

  • D'AMICO, G; DUMITRACHE, MO; SIROKY, P; ALBRECHTOVA, K; SLOBODA, M; DOMSA, C; SANDOR, AD; BALAZSI, R; KANYARI, PWN; MODRY, D; MIHALCA, AD, 2015:Altitudinal and seasonal differences of tick communities in dogs from pastoralist tribes of Northern Kenya. VETERINARY PARASITOLOGY 212 , p. 318 - 323.
  • GOMEZ, A; PETRZELKOVA, K; YEOMAN, CJ; VLCKOVA, K; MRAZEK, J; KOPPOVA, I; CARBONERO, F; ULANOV, A; MODRY, D; TODD, A; TORRALBA, M; NELSON, KE; GASKINS, HR; WILSON, B; STUMPF, RM; WHITE, BA; LEIGH, SR, 2015:Gut microbiome composition and metabolomic profiles of wild western lowland gorillas (Gorilla gorilla gorilla) reflect host ecology. MOLECULAR ECOLOGY 24 (10), p. 2551 - 2565.
  • JURANKOVA, J; BASSO, W; NEUMAYEROVA, H; FRENCOVA, A; BALAZ, V; DEPLAZES, P; KOUDELA, B, 2015:Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection. FOOD MICROBIOLOGY 52 , p. 150 - 153.
  • LAWRENCE, AL; HII, SF; JIRSOVA, D; PANAKOVA, L; IONICA, AM; GILCHRIST, K; MODRY, D; MIHALCA, AD; WEBB, CE; TRAUB, RJ; SLAPETA, J, 2015:Integrated morphological and molecular identification of cat fleas (Ctenocephalides felis) and dog fleas (Ctenocephalides canis) vectoring Rickettsia felis in central Europe. VETERINARY PARASITOLOGY 210 (42097), p. 215 - 223.

2014

  • FLAHOU, B; MODRY, D; POMAJBIKOVA, K; PETRZELKOVA, KJ; SMET, A; DUCATELLE, R; PASMANS, F; SA, RM; TODD, A; HASHIMOTO, C; MULAMA, M; KIANG, J; ROSSI, M; HAESEBROUCK, F, 2014:Diversity of zoonotic enterohepatic Helicobacter species and detection of a putative novel gastric Helicobacter species in wild and wild-born captive chimpanzees and western lowland gorillas. VETERINARY MICROBIOLOGY 174 (42036), p. 186 - 194.
  • HASEGAWA, H; MODRY, D; KITAGAWA, M; SHUTT, KA; TODD, A; KALOUSOVA, B; PROFOUSOVA, I; PETRZELKOVA, KJ, 2014:Humans and Great Apes Cohabiting the Forest Ecosystem in Central African Republic Harbour the Same Hookworms. PLOS NEGLECTED TROPICAL DISEASES 8 (3)
  • JANATOVA, M; ALBRECHTOVA, K; PETRZELKOVA, KJ; DOLEJSKA, M; PAPOUSEK, I; MASARIKOVA, M; CIZEK, A; TODD, A; SHUTT, K; KALOUSOVA, B; PROFOUSOVA-PSENKOVA, I; MODRY, D; LITERAK, I, 2014:Antimicrobial-resistant Enterobacteriaceae from humans and wildlife in Dzanga-Sangha Protected Area, Central African Republic. VETERINARY MICROBIOLOGY 171 (42097), p. 422 - 431.
  • KALOUSOVA, B; PIEL, AK; POMAJBIKOVA, K; MODRY, D; STEWART, FA; PETRZELKOVA, KJ, 2014:Gastrointestinal Parasites of Savanna Chimpanzees (Pan troglodytes schweinfurthii) in Ugalla, Tanzania. INTERNATIONAL JOURNAL OF PRIMATOLOGY 35 (2), p. 463 - 475.
  • KVAC, M; HOFMANNOVA, L; HLASKOVA, L; KVETONOVA, D; VITOVEC, J; MCEVOY, J; SAK, B, 2014:Cryptosporidium erinacei n. sp (Apicomplexa: Cryptosporidiidae) in hedgehogs. VETERINARY PARASITOLOGY 201 (42036), p. 9 - 17.
  • PROMEROVA, M; ANDERSSON, LS; JURAS, R; PENEDO, MCT; REISSMANN, M; TOZAKI, T; BELLONE, R; DUNNER, S; HORIN, P; IMSLAND, F; IMSLAND, P; MIKKO, S; MODRY, D; ROED, KH; SCHWOCHOW, D; VEGA-PLA, JL; MEHRABANI-YEGANEH, H; YOUSEFI-MASHOUF, N; COTHRAN, EG; LINDGREN, 2014:Worldwide frequency distribution of the 'Gait keeper' mutation in the DMRT3 gene. ANIMAL GENETICS 45 (2), p. 274 - 282.
  • SAK, B; PETRZELKOVA, KJ; KVETONOVA, D; MYNAROVA, A; POMAJBIKOVA, K; MODRY, D; CRANFIELD, MR; MUDAKIKWA, A; KVAC, M, 2014:Diversity of Microsporidia, Cryptosporidium and Giardia in Mountain Gorillas (Gorilla beringei beringei) in Volcanoes National Park, Rwanda. PLOS ONE 9 (11)

2013

  • POMAJBIKOVA, K; OBORNIK, M; HORAK, A; PETRZELKOVA, KJ; GRIM, JN; LEVECKE, B; TODD, A; MULAMA, M; KIYANG, J; MODRY, D, 2013:Novel Insights into the Genetic Diversity of Balantidium and Balantidium-like Cyst-forming Ciliates. PLOS NEGLECTED TROPICAL DISEASES 7 (3)
  • SCHOVANCOVA, K; POMAJBIKOVA, K; PROCHAZKA, P; MODRY, D; BOLECHOVA, P; PETRZELKOVA, KJ, 2013:Preliminary Insights into the Impact of Dietary Starch on the Ciliate, Neobalantidium coli, in Captive Chimpanzees. PLOS ONE 8 (11)

GRANTY

  • Genetická diverzita a přenosy strongylidních hlístic mezi africkými lidoopy a lidmi (15-05180S), Czech Science Foundation - Standard Grants, 2015 - 2017
  • Impact of increased contact with humans on diversity and ecology of protozoan parasites of African great apes (GA206/09/0927), Czech Science Foundation - Standard Grants, 2009 - 2011
  • Development of the new tools for surveillance of trichinellosis in domestic swine and wildlife animals in the Czech Republic (QH81069), Ministry of Agriculture - The Research Program in the Agricultural Sector, 2008 - 2012
  • Immunogenetic study of a house mouse hybrid zone (GA206/08/0640), Czech Science Foundation - Standard Grants, 2008 - 2012

The laboratories are well equipped to perform molecular-genetic diagnosis of parasites and advanced microscopy diagnosis.

Přečtěte si také
Educational Course: NeuroImaging - Mapping the function and structure of brain

19. září 2017 14:37

Educational Course: NeuroImaging - Mapping the function and structure…

WHEN: November 14-16, 2017 WHERE: CF MAFIL, CEITEC MU, University Campus Bohunice, Brno

Mladá laborantka z CEITEC VUT zvítězila v mezinárodní posterové soutěži

19. září 2017 9:15

Mladá laborantka z CEITEC VUT zvítězila v mezinárodní posterové…

Markéta Tesařová je sice teprve v magisterském studiu oboru Fyzikální inženýrství a nanotechnologie FSI VUT, ale už sbírá úspěchy i v oblasti…