Změnit instituci
Pokročilé nano a mikrotechnologie Pokročilé materiály Strukturní biologie Gen. a prot. rostlin. systémů Molekulární medicína Výzkum mozku a lidské mysli Molekulární vet. medicína

Experimentální biofotonika

Vedoucí výzkumné skupiny
Researcher ID
Telefon: +420 54114 2795, +420 604 072 795
Mobil: +420 604 072 795
E-mail:
Kancelář:
Zástupce vedoucího výzkumné skupiny
Researcher ID
E-mail:
Kancelář:

Research areas

  • advanced light (holographic, multimodal) microscopy (ALM) – innovative technologiesCzechBioImaging
  • evaluation of live cell behaviour using ALM
  • analysis of micro- and nanostructures using ALM

Main objectives

Research and development of analytical and measurement methods

Experimental Biophotonics focus on advanced light microscopy techniques and methodologies applied to the study of nano- and microstructures characteristics and interactions with living cells including assessments of possible cytotoxicity and cancer cell diagnostics.

Content of research

Advanced light microscopy – innovative technologies

  • theoretical study of imaging processes in advanced, especially holographic and confocal light microscopy, simulation and experimental verification of results
  • investigation of coherence effects, optical and coherence vortices in holographic imaging, imaging in turbid media by coherence gating
  • development of coherence controlled and SLM optical systems for holographic and multimodal (correlative) microscopy
  • development of advanced image-processing methods for holographic reconstruction, quantitative phase evaluation, dynamic phase differences, pattern and process recognition

Evaluation of live cell behaviour & analysis of micro- and nanostructures

  • biophysics of quantitative evaluation of live cell behaviour developed with coherence- controlled holographic microscopy (CCHM)
  • subtoxic impact/toxicity of nanomaterials assessment by measurement of intracellular dry mass translocation with CCHM
  • in vitro qualification of cancer cell malignancy with CCHM classification of cell behaviour patterns
  • observation and validation of nanostructure properties and their interactions with living cells with advanced light microscopy

Research group SITE

Research group FACEBOOK profile

seznam / vizitky

Jméno a pozice

E-mail

Telefon

Ing. Matěj Týč, Ph.D.
Junior researcher
+420 54114 2789
Mgr. Alena Vojkůvková
Administrativní pracovnice
+420 54114 2833
MUDr. Pavel Veselý, CSc.
Senior researcher
Ing. Lukáš Kvasnica, Ph.D.
Junior researcher
+420 54114 2816
prof. RNDr. Jiří Komrska, CSc.
Senior researcher
+420 54114 2826
Ing. Pavel Kolman, Ph.D.
Junior researcher
+420 54114 2816, +420 723 124 504
Ing. Hana Uhlířová, Ph.D.
Junior researcher
+420 54114 2833
prof. RNDr. Jiří Petráček, Dr.
Senior researcher
+420 54114 2764, +420 726 812 764
Ing. Martin Antoš, Ph.D.
Junior researcher
+420 54114 2778, +420 726 812 778
Ing. Petr Bouchal, Ph.D.
Výzkumný pracovník junior
+420 54114 2778
Ing. Jana Čolláková
Junior researcher
+420 54114 2789, +420 777 658 094
Ing. Zbyněk Dostál, Ph.D.
junior researcher
+420 54114 2833, +420 54114 2778
prof. RNDr. Radim Chmelík, Ph.D.
Senior researcher
+420 54114 2795, +420 604 072 795
Ing. Lenka Štrbková
Ph.D. student
+420 54114 2789
Ing. Daniel Zicha, CSc.
senior researcher
Mgr. Veronika Jůzová
Technický pracovník - laborant
Ing. Michala Slabá
Ph.D. student
+420 54114 2833
Ing. Aneta Křížová
Ph.D. student
+420 54114 2789

VYBRANÉ PUBLIKACE

2017

  • BABOCKY, J; KRIZOVA, A; STRBKOVA, L; KEJIK, L; LIGMAJER, F; HRTON, M; DVORAK, P; TYC, M; COLLAKOVA, J; KRAPEK, V; KALOUSEK, R; CHMELIK, R; SIKOLA, T, 2017:Quantitative 3D Phase Imaging of Plasmonic Metasurfaces. ACS PHOTONICS 4 (6), p. 1389 - 1397.
  • UHLÍŘOVÁ, H; PEIFANG, T; KILIC, K; THUNEMANN, M; SRIDHAR, V; BARTSCH, H; DALE, A; DEVOR, A; SAISAN, P, 2017:Neurovascular Network Explorer 2.0: A Database of 2-Photon Single Vessel Diameter Measurements from Mouse SI Cortexin Response To Optogenetic Stimulation. Frontiers in Neuroinformatics (11)

2016

  • STRBKOVA, L; MANAKHOV, A; ZAJICKOVA, L; STOICA, A; VESELY, P; CHMELIK, R, 2016:The adhesion of normal human dermal fibroblasts to the cyclopropylamine plasma polymers studied by holographic microscopy. SURFACE & COATINGS TECHNOLOGY 295 , p. 70 - 77.
  • UHLIROVA, H; KILIC, K; TIAN, PF; SAKADZIC, S; GAGNON, L; THUNEMANN, M; DESJARDINS, M; SAISAN, PA; NIZAR, K; YASEEN, MA; HAGLER, DJ; VANDENBERGHE, M; DJUROVIC, S; ANDREASSEN, OA; SILVA, GA; MASLIAH, E; KLEINFELD, D; VINOGRADOV, S; BUXTON, RB; EINEVOLL, GT; BOAS, DA; DALE, AM; DEVOR, A, 2016:The roadmap for estimation of cell-type-specific neuronal activity from non-invasive measurements. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 371 (1705)
  • UHLIROVA, H; KILIC, K; TIAN, PF; THUNEMANN, M; DESJARDINS, M; SAISAN, PA; SAKADZIC, S; NESE, TV; MATEO, C; CHENG, Q; WELDY, KL; RAZOUX, F; VANDENBERGHE, M; CREMONESI, JA; FERRI, CGL; NIZAR, K; SRIDHAR, VB; STEED, TC; ABASHIN, M; FAINMAN, Y; MASLIAH, E; DJUROVIC, S; ANDREASSEN, OA; SILVA, GA; BOAS, DA; KLEINFELD, D; BUXTON, RB; EINEVOLL, GT; DALE, AM; DEVOR, A, 2016:Cell type specificity of neurovascular coupling in cerebral cortex. ELIFE 5
  • ZEMANOVA, J; HYLSE, O; COLLAKOVA, J; VESELY, P; OLTOVA, A; BORSKY, M; ZAPRAZNA, K; KASPARKOVA, M; JANOVSKA, P; VERNER, J; KOHOUTEK, J; DZIMKOVA, M; BRYJA, V; JASKOVA, Z; BRYCHTOVA, Y; PARUCH, K; TRBUSEK, M, 2016:Chk1 inhibition significantly potentiates activity of nucleoside analogs in TP53-mutated B-lymphoid cells. ONCOTARGET 7 (38), p. 62091 - 62106.
  • ZEMANOVA, J; HYLSE, O; COLLAKOVA, J; VESELY, P; OLTOVA, A; PARUCH, K; TRBUSEK, M, 2016:CHECKPOINT KINASE 1 INHIBITION POTENTIATES APOPTOSIS AND INFLICTS MITOTIC FAILURE IN CLL-DERIVED MEC-1 CELL LINE. HAEMATOLOGICA 101 , p. 425 - 425.

2015

  • COUVRAT-DESVERGNES, G; SALAMA, A; LE BERRE, L; EVANNO, G; VIKLICKY, O; HRUBA, P; VESELY, P; GUERIF, P; DEJOIE, T; ROUSSE, J; NICOT, A; BACH, JM; ANG, E; FOUCHER, Y; BROUARD, S; CASTAGNET, S; GIRAL, M; HARB, J; PERREAULT, H; CHARREAU, B; LORENT, M; SOULILLOU, JP, 2015:Rabbit antithymocyte globulin-induced serum sickness disease and human kidney graft survival. JOURNAL OF CLINICAL INVESTIGATION 125 (12), p. 4655 - 4665.
  • KRIZOVA, A; COLLAKOVA, J; DOSTAL, Z; KVASNICA, L; UHLIROVA, H; ZIKMUND, T; VESELY, P; CHMELIK, R, 2015:Dynamic phase differences based on quantitative phase imaging for the objective evaluation of cell behavior. JOURNAL OF BIOMEDICAL OPTICS 20 (11)
  • PLOSCHNER, M; KOLLAROVA, V; DOSTAL, Z; NYLK, J; BARTON-OWEN, T; FERRIER, DEK; CHMELIK, R; DHOLAKIA, K; CIZMAR, T, 2015:Multimode fibre: Light-sheet microscopy at the tip of a needle. SCIENTIFIC REPORTS 5
  • SALAMA, A; COUVRAT-DESVERGNES, G; LE BERRE, L; EVANNO, G; VIKLICKY, O; HRUBA, P; VESELY, P; GUERIF, P; DEJOIE, T; NICOT, AB; BACH, JM; BROUARD, S; CASTAGNET, S; GIRAL, M; HARB, J; FOUCHER, Y; LORENT, M; SOULILLOU, JP, 2015:INCREASED LATE GRAFT LOSS IN KIDNEY RECIPIENTS WITH SERUM SICKNESS DISEASE FOLLOWING ANTI-THYMOCYTE GLOBULIN INDUCTION: RELATION WITH AN ANTI-NEU5GC RESPONSE. TRANSPLANTATION 99 (11), p. S137 - S138.
  • TRUNEC, M; MACA, K; CHMELIK, R, 2015:Polycrystalline alumina ceramics doped with nanoparticles for increased transparency. JOURNAL OF THE EUROPEAN CERAMIC SOCIETY 35 (3), p. 1001 - 1009.

2014

  • BOUCHAL, P; BOUCHAL, Z, 2014:Non-iterative holographic axial localization using complex amplitude of diffraction-free vortices. OPTICS EXPRESS 22 (24), p. 30200 - 30216.
  • BUSBY, SA; ROBB, A; LANG, S; TAKEUCHI, Y; VESELY, P; SCOBIE, L, 2014:Antibiotic susceptibility and resistance of Staphylococcus aureus isolated from fresh porcine skin xenografts: Risk to recipients with thermal injury. BURNS 40 (2), p. 288 - 294.
  • LOST'AK, M; CHMELIK, R; SLABA, M; SLABY, T, 2014:Coherence-controlled holographic microscopy in diffuse media. OPTICS EXPRESS 22 (4), p. 4180 - 4195.
  • MESTAK, O; MATOUSKOVA, E; SPURKOVA, Z; BENKOVA, K; VESELY, P; MESTAK, J; MOLITOR, M; POMBINHO, A; SUKOP, A, 2014:Mesenchymal Stem Cells Seeded on Cross-Linked and Noncross-Linked Acellular Porcine Dermal Scaffolds for Long-Term Full-Thickness Hernia Repair in a Small Animal Model. ARTIFICIAL ORGANS 38 (7), p. 572 - 579.

2013

  • BOUCHAL, P; BOUCHAL, Z, 2013:Concept of coherence aperture and pathways toward white light high-resolution correlation imaging. NEW JOURNAL OF PHYSICS 15
  • BOUCHAL, P; BOUCHAL, Z, 2013:Wide-field common-path incoherent correlation microscopy with a perfect overlapping of interfering beams. JOURNAL OF THE EUROPEAN OPTICAL SOCIETY-RAPID PUBLICATIONS 8 (13011)
  • MATOUSKOVA, M; VESELY, P; DANIEL, P; MATTIUZZO, G; HECTOR, RD; SCOBIE, L; TAKEUCHI, Y; HEJNAR, J, 2013:Role of DNA Methylation in Expression and Transmission of Porcine Endogenous Retroviruses. JOURNAL OF VIROLOGY 87 (22), p. 12110 - 12120.
  • NIZAR, K; UHLIROVA, H; TIAN, PF; SAISAN, PA; CHENG, Q; REZNICHENKO, L; WELDY, KL; STEED, TC; SRIDHAR, VB; MACDONALD, CL; CUI, JX; GRATIY, SL; SAKADZIC, S; BOAS, DA; BEKA, TI; EINEVOLL, GT; CHEN, J; MASLIAH, E; DALE, AM; SILVA, GA; DEVOR, A, 2013:In vivo Stimulus-Induced Vasodilation Occurs without IP3 Receptor Activation and May Precede Astrocytic Calcium Increase. JOURNAL OF NEUROSCIENCE 33 (19), p. 8411 - 8422.
  • ROSEL, D; BRABEK, J; VESELY, P; FERNANDES, M, 2013:Drugs for solid cancer: the productivity crisis prompts a rethink. ONCOTARGETS AND THERAPY 6 , p. 767 - 777.
  • SCOBIE, L; PADLER-KARAVANI, V; LE BAS-BERNARDET, S; CROSSAN, C; BLAHA, J; MATOUSKOVA, M; HECTOR, RD; COZZI, E; VANHOVE, B; CHARREAU, B; BLANCHO, G; BOURDAIS, L; TALLACCHINI, M; RIBES, JM; YU, H; CHEN, X; KRACIKOVA, J; BROZ, L; HEJNAR, J; VESELY, P; TAKEUC, 2013:Long-Term IgG Response to Porcine Neu5Gc Antigens without Transmission of PERV in Burn Patients Treated with Porcine Skin Xenografts. JOURNAL OF IMMUNOLOGY 191 (6), p. 2907 - 2915.
  • SLABY, T; KOLMAN, P; DOSTAL, Z; ANTOS, M; LOST'AK, M; CHMELIK, R, 2013:Off-axis setup taking full advantage of incoherent illumination in coherence-controlled holographic microscope. OPTICS EXPRESS 21 (12), p. 14747 - 14762.
  • TYC, M; KVASNICA, L; SLABA, M; CHMELIK, R, 2013:Numerical refocusing in digital holographic microscopy with extended-sources illumination. OPTICS EXPRESS 21 (23), p. 28258 - 28271.

2012

  • ANTOS, M; COLLAKOVA, J; VESELY, P; CHMELIK, R; KRIZOVA, A, 2012:System for coherence-controlled holographic microscopy of living cells. 18TH CZECH-POLISH-SLOVAK OPTICAL CONFERENCE ON WAVE AND QUANTUM ASPECTS OF CONTEMPORARY OPTICS 8697
  • BOUCHAL, P; BOUCHAL, Z, 2012:Selective edge enhancement in three-dimensional vortex imaging with incoherent light. OPTICS LETTERS 37 (14), p. 2949 - 2951.
  • HENZLOVA, M; LOST'AK, M; CHMELIK, R, 2012:Theoretical study of coherence-controlled holographic microscopy in the presence of scattering media. 18TH CZECH-POLISH-SLOVAK OPTICAL CONFERENCE ON WAVE AND QUANTUM ASPECTS OF CONTEMPORARY OPTICS 8697
  • CHMELIK, R, 2012:Coherent Imaging with Incoherent Light in Digital Holographic Microscopy. 18TH CZECH-POLISH-SLOVAK OPTICAL CONFERENCE ON WAVE AND QUANTUM ASPECTS OF CONTEMPORARY OPTICS 8697

2011

  • CHMELIK, R, 2011:Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope. HOLOGRAPHY: ADVANCES AND MODERN TRENDS II 8074

2010

  • BRABEK, J; MIERKE, CT; ROSEL, D; VESELY, P; FABRY, B, 2010:The role of the tissue microenvironment in the regulation of cancer cell motility and invasion. CELL COMMUNICATION AND SIGNALING 8
  • LOST'AK, M; KOLMAN, P; DOSTAL, Z; CHMELIK, R, 2010:Diffuse light imaging with a coherence controlled holographic microscope. 17TH SLOVAK-CZECH-POLISH OPTICAL CONFERENCE ON WAVE AND QUANTUM ASPECTS OF CONTEMPORARY OPTICS 7746
  • TYC, M; CHMELIK, R, 2010:Numerical Refocusing of Planar Samples Unlimited. 17TH SLOVAK-CZECH-POLISH OPTICAL CONFERENCE ON WAVE AND QUANTUM ASPECTS OF CONTEMPORARY OPTICS 7746
  • ZIKMUND, T; KVASNICA, L; UHLIROVA, H; LOVICAR, L; CHMELIK, R, 2010:Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy. 17TH SLOVAK-CZECH-POLISH OPTICAL CONFERENCE ON WAVE AND QUANTUM ASPECTS OF CONTEMPORARY OPTICS 7746

GRANTY

  • Pokroky nekoherentní holografické mikroskopie při použití fotonických simulací a principů singulární optiky (GA15-14612S), Czech Science Foundation - Standard Grants, 2015 - 2017
  • Validation of digital holographic microscope in investigations of living cell dynamics & completion of new multifunctional instrument (GA202/08/0590), Czech Science Foundation - Standard Grants, 2008 - 2010
  • Image reconstruction of three-dimensional objects by the methods of the holographic confocal microscopy (GA202/04/1410), Czech Science Foundation - Standard Grants, 2004 - 2006
  • The significance of fast intracellular motile reactions in living cells (GA304/99/0368), Czech Science Foundation - Standard Grants, 1999 - 2001
  • Inorganic nanomaterials and nanostructures: fabrication, characterization, properties (MSM0021630508), MEYS - Institutional Research Plan, 2005 - 2011

CURRENT RESEARCH INFRASTRUCTURE

  • first generation of coherence-controlled holographic microscopes: transmitted-light system adapted for live-cell time-lapse observations (heated box, flow chambers, anti-vibration system), reflected-light system, image-processing SW for on-line holographic reconstruction and evaluation of quantitative phase contrast
  • microscopes: Nikon Eclipse TI A1R MP inverted confocal system (fluorescence, DIC, RIC, Tokai-Hit stage-top incubator), Nikon Eclipse TS100 inverted phase-contrast microscope, Nikon Eclipse L150 with reflected light (BF, DF, DIC, fluorescence microscopy), Nikon Alphaphot 2, Confocal 2002 tandem-scanning reflected-light confocal microscope, NIS elements image-processing SW
  • basic facilities for cell-culture (GMO approved) and sample preparation: biohazard box, thermal box, centrifuge Rotofix, phase-contrast microscope
  • complete equipment for research and development in the field of advanced light microscopy: optical design and simulation software, optical tables, opto-mechanical components, optical kits, halogen, xenon and laser sources, digital cameras, SLM
Přečtěte si také
Advanced Materials and Nanotechnology Seminar Series: Dr. Zoltan RACZ

22. září 2017 12:44

Advanced Materials and Nanotechnology Seminar Series: Dr. Zoltan RACZ

WHO? Dr. Zoltan Racz WHAT ABOUT? Paper-based Electronics – Materials, Fabrication and Applications WHEN? Tuesday: September, 26 starts 13:…

Lecture: Regulation of transcription elongation in E. coli by the RNA polymerase – RNA interactome

22. září 2017 10:34

Lecture: Regulation of transcription elongation in E. coli by the RNA…

WHEN: September 29, 2017 from 14:00 WHERE: Room 145, building A35, University Campus Bohunice SPEAKER: Prof. Renée Schroeder, …