Q-Phase holographic microscope (Q-Phase)

Q-Phase holographic microscope
CONTACT US

Guarantor: Zbyněk Dostál, Ph.D.
Instrument status: Operational Operational, 15.12.2022 12:56
Equipment placement: Vysoké učení technické v Brně, CEITEC
Research group: Biophotonics Core Facility


Description:

Q-Phase Multimodal holographic microscope (MHM) uses incoherent holography, which enables to acquire quantitative phase images with high lateral resolution comparable with conventional widefield microscopes.

Featured operating modes
- Transmission mode: suitable for biological and biotechnical applications (cell life cycle, cell growth, cell motility, morphological observations, etc.).
- Reflection mode: suited for applications from material science and topography studies with nanometer axial resolution.
- Fluorescence mode: enables additional comparative experiments

Advantages of MHM
- quantitative phase imaging (without halo effect)
- imaging of live cells in their native environment
- label-free, non-invasive observation
- observation in turbid media
- system located in an incubator (adjustable temperature)
- imaging without speckles and parasitic interferences (in comparison with laser-based HM)
- fast image acquisition (no scanning necessary)
- user-friendly system: motorized parts (objective exchange, sample stage, and focusing), automated microscope adjustment, easy operating with control panel, automated multidimensional acquisition (time-lapse, multichannel, Z-stack)

The system is available to the scientific public for their tests and applications.

The development of Multimodal Holographic Microscope is carried out in cooperation with TESCAN Brno, s.r.o. The worldwide unique technical solution is covered by EU, USA, and EA patents, patent pending in several other countries worldwide.